Anti-tumor and immunomodulatory effects of Grifola frondosa polysaccharide combined with vitamin C on Heps-bearing mice: Based on inducing apoptosis and autophagy

Abstract

• Grifola frondosa polysaccharide combined with vitamin C has the same obvious anti-tumor effect in mouse liver cancer models as in in vitro experiments, and the tumor inhibition rate exceeds 50%. The mechanism of action is to induce tumor apoptosis and autophagy at the same time. • The combined application of GFP/VC can also enhance the cellular immune function of mice by increasing the percentage of CD4 + CD8 + T cells,B cells and Treg cells. • The combined application of GFP/VC can also enhance the humoral immune function of mice by increasing the secretion of various cytokines (IL-2,IL-12p70,TNF-α and IFN-γ). The aim of this study was to detect the anti-tumor and immunomodulatory effects of Grifola frondosa polysaccharide (GFP) combined with Vitamin C (VC) on Heps-bearing Mice. The tumor inhibition rate of GFP/VC intragastric administration or intraperitoneal injection were 57.92% and 69.58% respectively. Immunohistochemical and Western Blot results showed the expression of apoptosis-related and autophagy-related proteins (PARP, Caspase3, Beclin-1 and LC-3) all increased. FCM results indicated that the percentages of CD4 + T, CD8 + T, B lymphocytes, and Treg cells in the GFP/VC treatment group all increased. And the secretion of GM-CSF, IL-4,IL-10 and MIP-3a/CCL20 in peripheral blood significantly reduced. On the contrary, the secretion of IFN-γ, TNF-α, IL-2 and IL-12p70 increased after GFP/VC treatment. And in the above indicators, the differences between the experimental group and the negative control group were statistically significant ( P all < 0.05). So GFP combined with VC have obvious anti-tumor and immunomodulatory effect in mouse liver cancer models.