Anti-Tumor Activity of Viper Snake Venom Photo-products SV1 and SV2 against Ehrlich Ascites Carcinoma in Mice.

Abstract

Objectives: To evaluate antitumor activity potential of viper venom photoproducts SV1 and SV2 against Ehrlich ascites carcinoma in mice. Materials and Methods: Viper venom photoproducts SV1 (Vipera russelli ) and SV2 (Echis carinatus) were generated by exposure of the venoms to UV sensitized methylene blue for 15 min. and 90 min. at 370C respectively. The Antitumor activity of SV1 and SV2 at two concentrations (1/10 th and 1/20th dilutions) was evaluated on Ehrlich Ascites Carcinoma (EAC) inoculated to Swiss male mice. The antitumor response was measured from tumor volume (TV), packed cell volume (PCV), body weight (BW), hematological parameters (Hb, RBC, total WBC) and biochemical parameters (serum LDH, ALT, AST and hepatic tissue total protein, reduced GSH and TBAR (MDA). Half of the animals (n=6) were used to determine the mean survival time (MST) and the percentage increase in the life span (%ILS). Results: The SV1 and SV2 decreased dose dependently TV, PCV, BW, WBC, serum levels of LDH, ALT; increased dose dependently serum AST level, hepatic total protein, reduced GSH, TBARs (MDA) and serum Hb in SV2A. The results were statistically significant. The % ILS at the 1/10th dilution of SV1 and SV2 was 40.27% and 29.52% respectively. Conclusion: SV1 and SV2 at 1/10 th dose level showed significant antitumor activity based on percent increase in the survival time, reduction in the tumor volume, packed cell volume and restoration of hematological and biochemical parameters towards normal value.