Abstract
BackgroundEuphorbia is the largest genus in family Euphorbiaceae with a great biological and genetic diversity. The anti-trypanosomal activity of the crude extract of 15 Euphorbia species against Trypanosoma brucei brucei was carried out. Furthermore, DNA fingerprinting of the tested species using ISSR and SCoT markers was also investigated.ResultsThe anti-trypanosomal activity of the 15 Euphorbia species revealed the highest activity of E. officinarum L. and E. milli Des Moul. against Trypanosoma brucei brucei with IC50 values < 10 μg/mL after 48- and 72-h incubation. Moreover, the assessment of the genetic diversity among the 15 tested species showed similar correlation coefficients of 0.76–0.98 which classified Euphorbia species into two main groups, one contained two species and the other contained 13 species.ConclusionsSome Euphorbia species exhibited significant growth inhibitory activity toward Trypanosoma brucei strain TC221. Results also indicated the suitability of both markers for genetic fingerprinting of the tested Euphorbia species. To our knowledge, this is the first detailed comparison of the performance of two targeted DNA molecular markers (SCoT and ISSR) on the tested 15 Euphorbia species. The results guide future efficient use of these molecular markers in the genetic analysis of Euphorbia.
Highlights
Euphorbia is the largest genus in family Euphorbiaceae with a great biological and genetic diversity
The current study investigated the activity of 15 Euphorbia species against T. brucei strain TC 221, and results indicated high activity of four species with IC50 values < 20 μg/mL after 48-h and 72-h incubation
Results of the present study revealed that both markers generated high numbers of polymorphic bands that could be used in the diagnostic fingerprinting of Euphorbia species
Summary
Euphorbia is the largest genus in family Euphorbiaceae with a great biological and genetic diversity. The anti-trypanosomal activity of the crude extract of 15 Euphorbia species against Trypanosoma brucei brucei was car‐ ried out. Human African trypanosomiasis, sleeping sickness, is a mortal vector-borne parasitic disease caused by Trypanosoma brucei transmitted by the tsetse fly (Glossina spp.), especially in sub-Saharan Africa [26]. Only a few drugs have been approved to treat human African trypanosomiasis as suramin, pentamidine, melarsoprol, eflornithine, and the combination of nifurtimox/ eflornithine. Discovering anti-trypanosomal drugs from natural sources is an urgent requirement. Euphorbia genus contained a wide variety of terpenoids, ranging from mono-, sesqui-, and diterpenes to triterpenoids and steroids [24].
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