Abstract
Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata. Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional half-leaf method. Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis. The tested compounds showed moderate inactivation effect, whereas the lycoricidine derivatives showed good protective effect. The protective inhibitory activity of compounds L1 (N-methyl-2,3,4-trimethoxylycoricidine) (60.8%) and L3 (N-methyl-2-methoxy-3,4-acetonidelycoricidine) (62.0%) was almost similar to the positive control, Ningnanmycin (66.4%). RT-PCR and Western-blot analysis displayed that compounds L1, L3, L5 (N-allyl-2,3,4-triallyloxylycoricidine) exhibited antiviral activity, which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression. Additionally, defensive enzyme activities confirmed that compound L1 could increase the activity of PAL, POD, SOD to improve disease resistance of tobacco.Graphical
Highlights
The viruses of plant cause significant loss in agriculture and horticulture and have the name ‘‘plant cancer’’ [1]
Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus (TMV) by the conventional halfleaf method
Lycoricidine derivatives were carried out the assay of effect on systemic infection of TMV by westernblot and RT-PCR analysis
Summary
The viruses of plant cause significant loss in agriculture and horticulture and have the name ‘‘plant cancer’’ [1]. A series of natural products from plants and synthesised chemical compounds with anti-TMV activities had been reported, such as seco-pregnane steroids [3], 3-acetonyl-3-hydroxyoxindole [4], 7-deoxy-trans-dihydronarciclasine (DDN) [5], eudesmanolides [6], quassinoids [7], sesquiterpenoids [8], phenanthroindolizidines and their analogues [9], b-carbolines alkaloids [10] and their derivatives [11]. Among those compounds, DDN attracted our attention. Anti-TMV Effects of Amaryllidaceae Alkaloids Isolated from the Bulbs of Lycoris radiata
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
