Anti-Quorum Sensing Effect of Salvadora Persica Against Enterococcus faecalis (ATCC 29212)

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Introduction: Quorum Sensing (QS) is a mechanism many bacteria use to manage their cooperative activities and physiological functions. The Fsr system in Enterococcus faecalis (ATCC 29,212) is an example of quorum sensing with a cell density-dependent two-component regulatory system mechanism. Several publications have shown that the Fsr system and proteases independently contribute to E. faecalis pathogenicity in various infection models. Objectives: There is currently no published research to determine the exact molecular ability of Salvadora persica on quorum-sensing genes. Therefore, this study aimed to determine the plant extracts that inhibit the expression of the quorum-sensing gene (FsrC). Methods: Different fractions of Salvadora persica were obtained using different solvents, including standard hexane, chloroform, ethyl acetate, n-butanol, ethyl alcohol, and water which are expressed as fractions 1,2,3,4,5 and 6, respectively. Antibacterial activity assay of different plant extracts (S. persica) was determined by minimum inhibitory concentration (MIC). Finally, the relative expression of the quorum-sensing (QS) gene was evaluated using a One-step quantitative RT-PCR PrimeScript™ RT-PCR Kit. Results: All fractions of S. persica showed antimicrobial activity. However, ethyl acetate- S. persica inhibited the growth of E. faecalis (ATCC 29,212) at the lowest concentration, which was 20mg/ml and the highest concentration inhibited the growth of E. faecalis (ATCC 29,212) was 60mg/ml (chloroform- S. persica). Furthermore, the highest change fold value of (4.99) was recorded in treated E. faecalis (ATCC 29,212) with fraction 1 (hexane). Conclusion: Overall, S. persica showed antimicrobial activity against E. faecalis (ATCC 29,212). However, more studies are required to investigate the effect of different plant extracts on quorum-sensing genes of Enterococcus faecalis.