Abstract
Pseudomonas aeruginosa causes severe persistent infections in immunocompromised patients by showing tolerance conventional antibiotic therapy by virtue of specialized hierarchy of quorum sensing (QS) network. The purpose of the study was to evaluate the efficacy of two Mizoram medicinal plants i.e., Anogeissus acuminata and Mallotus roxburghianus Muell. in attenuating QS regulated P. aeruginosa virulence. The crude leaf extracts were tested for their ability to combat QS associated virulence phenotypes such as pyocyanin production, LasB elastase and Staphylolytic activity and production of biofilm determinants such as alginate and exopolysaccharide in P. aeruginosa PAO1. The gas chromatography mass spectrometry (GC-MS) analysis revealed the presence of phytocompounds, the synergistic activity of which is responsible for the anti QS activity. The in silico studies provide the binding efficacy of obtained phytochemical with QS receptor protein, LasR. The in vitro studies followed by in silico analysis demonstrated the efficacy of phytochemical of A. acuminata and M. roxburghianus Muell. in competitively inhibiting the binding of natural ligand with LasR and thereby altering production of virulence phenotypes. The efficacy in down regulating bacterial virulence shown by A. acuminata and M. roxburghianus Muell. provides promising alternatives to develop next generation anti-pathogenic agents.
Highlights
Microbial pathogens possess significant public health concern by showing multi drug resistance (MDR) to conventional antibiotics
The aim of the present study is to evaluate the efficacy of selected medicinal plants from Mizoram, India in combating P. aeruginosa PAO1 pathogenicity
Among the 25 plants screened with four different solvent system, methanolic extract of A. acuminata and ethanolic extract of M. roxburghianus Muell. were selected for further bioassays on the basis of zone of inhibition (S1)
Summary
Microbial pathogens possess significant public health concern by showing multi drug resistance (MDR) to conventional antibiotics. The Las system comprises of LasR transcriptional activator which operates through a signal molecule, N-3-oxo-dodecanoyl-homoserine lactone (C12-HSL) whereas RhlR, as transcriptional regulator activated by a signal molecule called Nbutanoyl-homoserine lactone (C4-HSL) constitute Rhl system Both LasI/R and RhlI/R systems are intriguingly correlated with each other and coordinate the expression of virulence phenotypes such as proteases, elastases, cytotoxic pyocyanin, exopolysachharides (EPS) and rhamnolipid (Aybey and Demirkan, 2016). The ability of P. aeruginosa to form biofilm aids as an arsenal to the bacteria to provide tolerance against conventional antimicrobial therapy In this context, targeting QS associated virulence could provide alternative strategies to combat QS regulated pathogenicity (Luo et al, 2016). The aim of the present study is to evaluate the efficacy of selected medicinal plants from Mizoram, India in combating P. aeruginosa PAO1 pathogenicity
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