Anti-putrefying properties of the aqueous extract of fresh leaves of Manihot esculenta (cassava) on dead laboratory Sprague Dawley rats

Abstract

The leaves of Manihot esculenta (ME) have been used by ancestors, apart from food, to preserve human bodies before burial. Formalin remains the chemical agent of choice in modern times to preserve corpses. However, cost, associated health hazards and recent public health concerns meant that researchers must explore alternative means of continuing this age-long process of preservation of human remains. The present study aimed to explore the effectiveness of extracts from cassava (Manihot esculenta) leaves in the preservation of dead remains of corpses. Aqueous extracts from fresh leaves of M. esculenta (MELE) were used to preserve euthanised rats and the internal organs were harvested after 7 days for histological analysis. The histological sections of the stomach and liver were compared with those of control rats treated with 6 ml of formalin (10%). The low and intermediate doses of MELE preserved the tissues of the dead rats beyond 7 days, as evidenced by histological sections of the stomach and liver. Concentrations of MELE between 300 mg/kg and 1500 mg/kg showed adequate potency in preserving dead rats compared to formalin. However, the dead rats treated with doses of MELE greater than 1500 mg/kg showed rapid putrefaction after 7 days.