Abstract
This study focused on the anti‐proliferative effect of ethylacetate (EtOAc) fraction of the herbal plant Orostachys japonicus. This study examined the effect of EtOAc fraction on human gastric cancer (AGS) cell proliferation and investigated the underlying mitogen‐activated protein kinases (MAPK) signaling pathways. The EtOAc fraction inhibited the proliferation of AGS cells by inducing cell cycle arrest as evidenced by propidium iodide (PI) labeling and RT‐PCR analysis. In the cell cycle analyses, treatment with the EtOAc fraction resulted in a marked increase of cells in the sub‐G1 and G2/M phases. RT‐PCR was performed to study the correlation between G2/M arrest and transcription of cell cycle control genes. The anti‐proliferative effect of EtOAc fraction was accompanied by inhibition of CDK1 and cyclin B1 mRNA. To understand the role of MAPK signaling in the anti‐proliferative effect of EtOAc fraction in AGS cells this study assessed EtOAc fraction‐treated AGS cells using antibodies against p38 MAPK (p38), c‐Jun N‐terminal kinase (JNK), and extracellular signal‐regulated kinase (ERK). MAPK such as p38, JNK, and ERK were analyzed by Western blotting. Treatment of AGS cells with the EtOAc fraction resulted in time‐ and dose‐dependent activation of p38, JNK, and ERK.
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