Abstract
Leaves of Adinandra nitida constitute a kind of flavonoid-rich plant food. In this study, camellianin A, the main flavonoid in the leaves of Adinandra nitida, was prepared and identified by high performance liquid chromatography-photodiode array detector-electrospray ionization mass spectrometry (HPLC-PDA-ESI/MS). In the anticancer assay, it was found camellianin A could inhibit the proliferation of the human hepatocellular liver carcinoma Hep G2 and human breast adenocarcinoma MCF-7 cell lines in a dose-dependent manner and induce the significant increase of the G0/G1 cell population. After treated by camellianin A, phosphatidylserine of Hep G2 and MCF-7 cells could translocate significantly to the surface of the membrane. The increase of an early apoptotic population of Hep G2 and MCF-7 cells was observed. It was concluded that camellianin A not only affected the progress of the cell cycle, but also induced cells to enter into apoptosis.
Highlights
Flavonoids have received great attention and have been studied extensively for their antioxidant, antibacterial, analgesic antitumor properties
By using HPLC-Photodiode Array Detector (PDA)-ESI/MS, the compound eluted at 5.93 min in the HPLC (Figure 2) was identified as camellianin A
To determine whether the Hep G2 and MCF-7 cells treated with camellianin A undergo the apoptosis pathway, the cell distribution in the cell cycle was examined by Propidium iodide (PI) staining
Summary
Flavonoids have received great attention and have been studied extensively for their antioxidant, antibacterial, analgesic antitumor properties. Adinandra nitida (Theaceae), a wild plant from South China, is a flavonoid-rich plant source. It is reported to have many curative effects, such as reducing blood pressure, as well as antibacterial, antioxidant, and analgesic properties [5]. According to our previous studies [6,7,8], the total flavonoid content in A. nitida leaves can be more than 20%. There is the possibility for large-scale use of camellianin A in the health food and phytopharmaceutical industry. A. nitida leaves have not been thoroughly studied. Camellianin A (Figure 1), the main flavonoid in A. nitida leaves, was separated and its apoptotic induction in human. Hep G2 and MCF-7 cells were investigated for the first time
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