Abstract
BackgroundCalotropis procera leaf is one of the plants commonly utilized in phytomedicine in Nigeria. The present investigation explored the use of the extracts on cell viability and apoptosis respectively. In this study, the expression of the Cluster of differentiation 146 (CD146) in the blood of lung cancer patients on regulatory T cells (Tregs) was determined. The antioxidant and anti-proliferative effects of methanol extracts of Caloropis procera leaf on lung cancer cell H1299 were investigated.MethodsFrom the flow cytometry, the expression of the CD146+ in the T cells were evaluated using the healthy patient, adenocarcinoma, squamous, and small cell lung cancer respectively. The apoptosis of granulocytes, monocytes, lymphocytes, CD4+, and Treg were determined by 7-amino-actinomycin D/Annexin V-Allophycocyanin (APC) staining during the resting stage and after 24 h respectively. Immunofluorescence was conducted. Cell viability assay, hydroxyl (OH), hydrogen peroxide (H2O2) and nitric oxide (NO) scavenging radicals were conducted. Reducing power and flavonoid content of Calotropis procera were investigated. The effect of the Calotropis procera at different concentrations at 24hrs was determined.ResultsFrom the flow cytometry, the expression of the CD146+ on the T cells includes 4.60 % in healthy patients, 10.10, 12.20, 9.80 % in adenocarcinoma, squamous and small cell lung cancer. The apoptosis of granulocytes, monocytes, lymphocytes, CD4+ and Treg were determined by 7-amino-actinomycin D/Annexin V-APC staining during the resting stage and after 24 h which indicate that apoptosis also occurred on Treg. Immunofluorescence shows the presence of CD146 in lung cancer patient’s tissues. The methanol extracts of Calotropis procera leaf have antioxidant and anti-proliferative effects. Methanol extract of Calotropis procera leaf reduced CD146 expression on blood cells at 24 h.ConclusionIncreased CD146 expression in the Treg of lung cancer patients indicates that it may be a possible target for the treatment of lung cancer by utilizing potent immunotherapy or natural products such as methanol extract of Calotropis procera leaf which may ameliorate the expression of CD146. Calotropis procera has antioxidant, inhibitory capacity on H1299 lung cancer cells, and the ability to scavenge OH, H2O2, and NO radicals. Hence, this investigation strengthens the phyto-medicinal properties of Calotropis procera.
Highlights
Lung cancer is a fatal and deadly disease with poor prognosis [1]
Flow cytometry for detection of Cluster of differentiation 146 (CD146) Blood samples were collected from patients which include healthy patients, adenocarcinoma, squamous lung cancer and small cell lung cancer patients respectively and the flow cytometry was used to detect the percentage of the CD146 expression on the Regulatory T cells (Tregs) (CD4+, CD25+, CD127 dim/-) respectively
The flow cytometry was used for the investigations which showed that the expression of the CD 146 + in the T cells includes 4.60 % in healthy patients, 10.10 %, 12.20 %, 9.80 % in adenocarcinoma, squamous and small cell lung cancer respectively (Fig. 2; Table 1)
Summary
Lung cancer is a fatal and deadly disease with poor prognosis [1]. It has a variation of histological subtypes, and is the most disastrous cancer globally. The major causes of lung cancer are still debatable. Lung cancer is more widespread in men, both in global and in most countries. Lung cancer death in China is moderately high compared to most countries. It is predictable that lung cancer death in China may rise by about 40 % between 2015 and 2030 [7]. The antioxidant and anti-proliferative effects of methanol extracts of Caloropis procera leaf on lung cancer cell H1299 were investigated
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
