Abstract
The aim of this study is to investigate on anticancer properties of crude skin extract from marine catfish Tachysurus dussumieri on human colon cancer cell line. The biochemical contents protein, lipid and carbohydrate were estimated to be 1.02, 2.96 and 3.26 mg/ml respectively. The protein profiles were analysed by NATIVE and SDS-PAGE, results indicated both high and low molecular weight proteins ranging from 135 to 25 kDa. The crude extract was tested for its anticancer activity against human colon cancer cell line (HT 29) by MTT assay, the Inhibition concentration (IC50) was obtained on 600 μg/ml at 24 h. Fluorescent microscopy was used to study the cell morphology, nuclear condensation, early and late apoptotic stages by definite staining methods. DNA fragmentation and flow cytometric results revealed that the skin extract induces chromatin condensation, apoptotic cell death and also induce cell cycle arrest at G0/G1 phase on colon cancer cell line.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
