Anti-Phototoxicity Effect of Phenolic Compounds from Acetone Extract of Entada phaseoloides Leaves via Activation of COX-2 and iNOS in Human Epidermal Keratinocytes.

Yanisa Mittraphab,Dongmei Wang,Maki Nagata,Kuniyoshi Shimizu,Masako Matsumoto,Yhiya Amen

doi:10.3390/molecules27020440

Yanisa Mittraphab, Dongmei Wang + Show 4 more

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https://doi.org/10.3390/molecules27020440

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Abstract

The extract from Entada phaseoloides was employed as active ingredients of natural origin into cosmetic products, while the components analysis was barely reported. Using LC-DAD-MS/qTOF analysis, eleven compounds (1–11) were proposed or identified from acetone extract of E. phaseoloides leaves (AE). Among them, six phenolic compounds, protocatechuic acid (2), 4-hydroxybenzoic acid (3), luteolin-7-O-β-d-glucoside (5), cirsimaritin (6), dihydrokaempferol (9), and apigenin (10), were isolated by various chromatographic techniques. Protocatechuic acid (2), epicatechin (4), and kaempferol (11) at a concentration 100 μM increased the HaCaT cells viability of the UVB-irradiated cell without any cytotoxicity effect and reduced the expression of COX-2 and iNOS inflammation gene. Moreover, compounds 2 and 4 could have potent effects on cell migration during wound closure. These results suggest that compounds 2, 4, and 11 from AE have anti-photoaging properties and could be employed in pharmaceutical and cosmeceutical products.

Highlights

Entada phaseoloides (Family: Fabaceae) is a huge evergreen that grows high in the tropical forest and is found in Africa, Asia, and Australia’s lowland coastal forests
Quercetin and luteolin were purchased from Sigma-Aldrich
Gallic acid, protocatechuic acid, 4-hydroxybenzoic Molecules 2022, 27, x FOR PEER REVaIcEiWd, epicatechin, luteolin-7-O-β-D-glucoside, luteolin, quercetin, dihydrokaemp6feorfo1l3, apigenin, and kaempferol were confirmed by comparison with authentic standard

Summary

Introduction

Entada phaseoloides (Family: Fabaceae) is a huge evergreen that grows high in the tropical forest and is found in Africa, Asia, and Australia’s lowland coastal forests. In Southeast Asia and tropical regions, E. phaseoloides is a well-known traditional medicinal plant. It has been claimed that E. phaseoloides is used as folk therapy for a variety of diseases and has a wide range of therapeutic characteristics. People in tropical and sub-tropical regions use almost all portions of this plant in traditional medicine to treat a wide range of diseases, including hemorrhoids, stomachaches, toothaches, spasms, gastritis, and lymphadenitis [1]. In 1989, the first report, E. phaseoloides leaves yielded entadamide C, a Sulphur-containing amide that was isolated and described [2]. The isolated compounds from E. phaseoloides seeds and stems such as quercetin, luteolin, apigenin, and dihydrokaempferol have been reported [3]. Entadine® is a photo-aging agent that shields the skin from both sun and indoor radiation It is a skin-care ingredient derived from the seeds and stems of E. phaseoloides

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