Anti-peptic Ulcer of the N-hexane Fraction from Sanchezia nobilis Hook.F. Leaves and its Isolated Compounds

Abstract

The n-hexane fraction of the Sanchezia nobilis Hook.f. leaves was evaluated for its effect on the gastric ulcer with pyloric ligation (Shay) rat model, analgesia effect on hot plate model and pain threshold meter, and inhibitory effect on Helicobacter pylori (H.P). The data showed a significant reduction in ulcer score, gastric volume, and total acidity and tended to reduce free acidity and increase gastric pH. In addition, the n-hexane fraction had a central analgesic effect and H.P inhibition. Chemical investigation of the n-hexane fraction resulted in the isolation of the four compounds (1 – 4). By using NMR and ESI-MS spectra, these compounds were identified chemical structures (3β, 5α, 22E)-stigmasta-7,22-dien-3-ol (1), coccinic acid (2), 7-hydroxy-6-methoxy coumarin (3), and betulinic acid (4). All compounds were reported for the first time from the Sanchezia genus.