Anti‐oxidative activity of db mice treated with glycosaminoglycan of cricket, Gryllus bimaculatus

Abstract

Gryllus bimaculatus (Gb, cricket) is newly emerged as an edible insect in several countries. Edible or medicinal insects have been employed by purification and identification of potent active substances as food or drugs. Anti‐diabetic activities of G. bimaculatus glycosaminoglycan (GbG) or dung beetle (Catharsius molossus) glycosaminoglycan (CaG) were evaluated in diabetic mice based on their abilities to reduce glucose, ALT, AST, LDL‐cholesterol, and BUN levels. Db mice were assigned into the following groups: 1) Db heterozygous (PBS, normal control), 2) Db homozygous (PBS, diabetes control), 3) CaG (5 mg/kg), 4) GbG (5 mg/kg), and 5) metformin (10 mg/kg). They were orally administered with PBS or CaG or GbG or metformin for one month. Sero‐biochemical levels were evaluated to determine their anti‐diabetic, and anti‐oxidative activities after one month of CaG or GbG treatment in Db mice. Blood glucose level was decreased after treatment with CaG or GbG. GbG produced meaningful anti‐diabetic effect. It inhibited LDL‐cholesterol and alkaline phosphatase levels. Regarding oxidative damage, levels of hepatocellular biomarkers levels and protein carbonyl content were reduced in db mice treated with CaG or GbG. Anti‐oxidative activities of catalase, superoxide dismutase, and glutathione peroxidase were significantly increased in GbG treated group compared to those in the control. Therefore, G. bimaculatus glycosaminoglycan could be used as a therapeutic agent with anti‐diabetic effect and anti‐oxidative activity for diabetic mice.Support or Funding InformationThis work was supported by Rural Development Administration basic Research project, PJ011853.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.