Anti-oxidant, anti-diabetic and anti-lipidemic activities of root bark extracts from Rhus tripartitum and cytotoxicity evaluation of isolated compounds

Abstract

This study was undertaken to investigate anti-oxidant, anti-diabetic and anti-lipidemic activities of three extracts from Rhus tripartitum root bark prepared by solvents with increasing polarity (hexane, ethyl acetate and n-butanol).Our result demonstrated that the n-butanol extract is endowed with the highest phenolic content 872.503 ± 0.788 mg (GAE)/g Extract, flavonoid content 325.19±0.294 mg(QE)/g Extract and tannin content 698.99± 0.605 mg(CE)/g Extract. In addition this extract was found to exhibit the highest anti-oxidant activity using DPPH radical-scavenging (IC50 = 0.008 mg/ml), ferric-reducing power (IC50=0.844 mg/ml), total antioxidant capacity concentration (756.758±0.940 mg (GAE)/g Extract) and nitric oxide radical scavenging (IC50 = 0.01 mg/ml) assays. Mean while, the ethyl acetate extract present the best anti-diabetic activity (30.11%) and anti-lipidemic activity (49.79%). Three compounds were isolated for the first time from the ethyl acetate extract of Rhus tripartitum and identified using extensive spectral techniques (MS, NMR 1D and 2D). The in vitro cytotoxic activity of isolated compounds on MCF-7 cells positive breast cancer cell line was investiguated. One of the isolated compounds present the interesting growth inhibitory effect on MCF-7 cell.