Anti-Outer membrane protein C and anti-glycoprotein 2 antibodies in inflammatory bowel disease and their association with complicated forms of Crohn's disease.

Darina Kohoutova,Stanislav Rejchrt,Jan Bures,Paula Moravkova,Marcela Drahosova

doi:10.1186/s12876-014-0190-1

Darina Kohoutova, Stanislav Rejchrt + Show 3 more

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https://doi.org/10.1186/s12876-014-0190-1

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Abstract

BackgroundPrecise diagnostics of inflammatory bowel disease (IBD) and identification of potentially more aggressive phenotypes of Crohn’s disease (CD) is urgently needed. The aim of our prospective study was to assess the relationship between serum anti-OmpC IgA (Outer membrane protein C), anti-GP2 (anti-glycoprotein 2) IgG and anti-GP2 IgA antibodies with IBD and their association with complicated forms of CD.MethodsThe study included 86 patients with CD, 25 patients with UC and 45 controls, blood donors. In CD group, 24/86 (28%) had B1 phenotype, 20/86 (23%) B2, 13/86 (15%) B3 and 29/86 (34%) B2 + B3. L1 involvement was present in 13/86 (15%), L2 in 13/86 (15%), L3 in 60/86 (70%). Serum anti-OmpC IgA, anti-GP2 IgG and IgA antibodies were investigated by means of ELISA. The data obtained were tested statistically by means of descriptive statistics, non-paired t-test, Mann-Whitney rank sum test, Spearman rank order correlation and Pearson product moment correlation using SigmaStat software.ResultsAnti-OmpC IgA were noted to be significantly higher in CD (median 32.6, inter-quartile range (IQR) 18.9-60.7) compared to the controls (median 18.3, IQR 11.1-23.1), p < 0.001. Anti-GP2 IgG were significantly higher in CD (median 13.9, IQR 8.6-25.6) compared to the controls (median 8.0, IQR 4.7-10.8), p < 0.001. Anti-GP2 IgA were significantly higher in CD (median 20.1, IQR 9.1-40.4) compared to the controls (median 9.8, IQR 5.6-16.9), p < 0.001. Significant difference was found in anti-OmpC IgA between UC (median 26.2, IQR 20.2-36.4) and the controls (median 18.3, IQR 11.1-23.1), p < 0.001. In CD anti-OmpC IgA were significantly higher in B2 compared to B1: p = 0.041 and in B2 + B3 compared to B1: p = 0.036. Anti-GP2 IgA were significantly higher in B2 + B3 compared to B1: p = 0.009 and in B3 compared to B1: p = 0.029. In CD there was a significant difference in anti-OmpC IgA between patients with surgery and without surgery, p = 0.005.ConclusionsWe have confirmed association between anti-OmpC IgA and IBD (CD and UC) and an association between anti-GP2 (IgG and IgA) and CD. Patients with complicated forms of CD have significantly higher levels of anti-OmpC IgA and anti-GP2 IgA.

Highlights

Precise diagnostics of inflammatory bowel disease (IBD) and identification of potentially more aggressive phenotypes of Crohn’s disease (CD) is urgently needed
In CD there was a significant correlation between antiGP2 IgG and anti-GP2 IgA values (r = 0.565, p < 0.001), between anti-GP2 IgG and anti-OmpC IgA values (r = 0.358, p < 0.001), between anti-GP2 IgA and antiOmpC IgA values (r = 0.385, p < 0.001)
Anti-OmpC antibodies are aimed at porins, proteins embedded in the outer membrane of Escherichia coli [20]

Summary

Introduction

Precise diagnostics of inflammatory bowel disease (IBD) and identification of potentially more aggressive phenotypes of Crohn’s disease (CD) is urgently needed. That there is an association between disease onset, location, behaviour and serologic markers, from which some do reflect contribution of large intestinal microbiota to the development of CD [8,9,10,11,12,13,14,15,16]. A study performed by Papp et al showed that the presence of auto-antibodies (including anti-OmpC) is associated with more complicated behaviour of the disease and the need for surgery in CD patients [11]. Pancreatic antibodies have been isolated more recently and association mainly with CD patients has been shown [14]

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