Abstract
Osteoporosis is a porous bone disease caused by bone density loss, which increases the risk of fractures. Cornus officinalis (CO) and Achyranthes japonica (AJ) have been used as traditional herbal medicine for various disorders in East Asia. Although the anti-osteoporotic effects of single extract of CO and AJ have already been reported, the synergistic effect of a combined mixture has not been studied. In this study, we investigated the effects of a CO and AJ herbal mixture on osteoporosis in in vitro and in vivo models. The results demonstrate that treatment with the CO and AJ mixture significantly promoted osteoblast differentiation of MC3T3-E1 mouse preosteoblasts through the upregulation of osteoblastic differentiation-associated genes such as alkaline phosphatase (Alpl), runt-related transcription factor 2 (Runx2), and bone gamma-carboxyglutamic acid-containing protein (Bglap), while the mixture significantly inhibited differentiation of osteoclasts isolated from primary-cultured mouse monocytes. In addition, oral administration of CO and AJ mixture significantly prevented bone mineral density loss and trabecular bone structures in an ovariectomy-induced osteoporotic mouse model. These results suggest that the combination treatment of CO and AJ mixture might be a beneficial therapy for osteoporosis.
Highlights
Bone is a specialized connective tissue which protects and supports various organs of the body [1]
Osteoblast differentiation plays an essential role in the control of bone formation that reduces the pathogenesis of osteoporosis [22]
To examine the effect of the Cornus officinalis (CO) and Achyranthes japonica (AJ) mixture on osteoblast differentiation, we evaluated the alkaline phosphatase (ALP) activity after CO and AJ mixture treatment, compared to single treatment in pre-osteoblast MC3T3-E1 cells
Summary
Bone is a specialized connective tissue which protects and supports various organs of the body [1]. The skeleton is involved in body homeostasis by providing structure and supporting organs, storing minerals, and producing blood cells [2]. A study reported that increased bone resorption by osteoclasts promotes bone mineral density (BMD) loss, leading to a high risk of the incidence of osteoporosis and bone fracture [7]. Several medications such as denosumab, bisphosphonates, and raloxifene have been recommended for osteoporosis treatment, inappropriate use of chemical agents has a significant risk of adverse effects and increased cost [8,9,10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
