Abstract
Osteoporosis is characterized by low bone density and quality with high risk of bone fracture. Here, we investigated anti-osteoporotic effects of natural plants (Lycii Radicis Cortex (LRC) and Achyranthes japonica (AJ)) in osteoblast and osteoclast cells in vitro and ovariectomized mice in vivo. Combined LRC and AJ enhanced osteoblast differentiation and mineralized bone-forming osteoblasts by the up-regulation of bone metabolic markers (Alpl, Runx2 and Bglap) in the osteoblastic cell line MC3T3-E1. However, LRC and AJ inhibited osteoclast differentiation of monocytes isolated from mouse bone marrow. In vivo experiments showed that treatment of LRC+AJ extract prevented OVX-induced trabecular bone loss and osteoclastogenesis in an osteoporotic animal model. These results suggest that LRC+AJ extract may be a good therapeutic agent for the treatment and prevention of osteoporotic bone loss.
Highlights
Bone is continuously remodeled and maintained by a balanced process between bone formation and resorption [1,2]
This study examined osteoprotective effects of Lycii radicis cortex (LRC) and Achyrantes japonica (AJ) in osteoblast and osteoclast cells and ovariectomized mice
Pre-osteoblastic cells were treated with three different concentrations (2, 10, and 50 μg/mL) of LRC and AJ extracts for 3 days, and bone formation enhancing effects were assessed by alkaline phosphatase (ALP) activity
Summary
Bone is continuously remodeled and maintained by a balanced process between bone formation and resorption [1,2]. Bone metabolism is a physiological condition that is controlled by depositing new bone formation by osteoblasts and resorbing old or damaged tissue by osteoclasts [1]. Osteoclasts break down or dissolve bone tissue and play a central role in the maintenance and remodeling of bone from vertebral skeleton. Nutrients 2019, 11, 2716 and formation leads to inappropriate bone remodeling, resulting in serious bone loss with bone metabolic diseases such as osteoporosis and osteopenia [3]. Osteoporosis in elderly or postmenopausal women is associated with aggressive bone resorption, leading to an enhanced risk of bone fragility and susceptibility to fractures [5]
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