Abstract
In this study, we first investigated the influence of Melandrium firmum Rohrbach (MF) on the accumulation of lipid content in 3T3‐L1 cells and in vitro results showed that MF extraction suppressed the differentiation of 3T3‐L1 pre‐adipocytes in a concentration‐dependent manner without showing cytotoxicity. Hence, we studied the effects of MF on preventing obesity in C57BL/6N mice. The results showed that MF decreased food efficiency ratio, body weight, epididymal adipose and hepatic tissue weight, hepatic lipid metabolites, and triacylglycerol and cholesterol serum levels, when compared with the high‐fat diet group. Moreover, MF significantly inhibited the expression of genes related to adipogenesis, such as PPAR‐γ, C/EBP‐α, and aP2, and those related to lipogenesis, such as SREBP‐1c, FAS, SCD‐1, and CD36 in epididymal adipose and liver tissues. These anti‐adipogenic and anti‐lipogenic effects of MF suggest that it could be used as a food including potential functional ingredient to prevent high‐fat diet‐induced obesity.
Highlights
In the industrialized world, obesity is one of the epidemics associated with an increased risk of other life-threatening pathologies (Ravinet Trillou et al, 2003)
Obesity is caused by the generation of new adipocytes (You, Lee, Kim, Kim, & Chang, 2014), and adipogenesis is a process of cell differentiation where pre-adipocytes mature into adipocytes (Wang, Hwang, Kim, & Lim, 2017)
We examined the anti-adipogenic effects of Melandrium firmum Rohrbach (MF) extract using Oil Red O staining in 3T3-L1 adipocytes
Summary
Obesity is one of the epidemics associated with an increased risk of other life-threatening pathologies (Ravinet Trillou et al, 2003). Obesity is caused by the generation of new adipocytes (You, Lee, Kim, Kim, & Chang, 2014), and adipogenesis is a process of cell differentiation where pre-adipocytes mature into adipocytes (Wang, Hwang, Kim, & Lim, 2017). Transcription factor family and peroxidase proliferator-activated receptor γ (PPAR γ) act to regulate adipocyte differentiation (Lee et al, 2010), sterol regulatory element-binding protein (SREBP-1c) acts as a stimulator (Ali, Hochfeld, Myburgh, & Pepper, 2013), and adipocyte P2(aP2) and fatty acid synthase (FAS) play a role in the maturation of adipocytes (Choi et al, 2000). The anti-obesity effects of the extract were investigated by measuring body weight gain, serum and lipid profiles, histological variation in the adipose tissue, and expression of the genes associated with adipogenesis and lipogenesis for 10 weeks in high-fat diet-fed obese mice
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