Anti-Obesity Effect of Hot Water Extract of Barley Sprout through the Inhibition of Adipocyte Differentiation and Growth.

Myeong-Jin Kim,Min-Jeong Kim,Young-Min Kim,Seung-Hee Jang,Ji-Won Seo,Jong-Tae Kim,Sang-Hyeon Kim,Hyo-Jae Lee,Hye-Won Kawk

doi:10.3390/metabo11090610

Abstract

Barley sprouts are known to have several effective physiological activities. In this study, the anti-obesity effect of a barley sprout hot water extract (BSE) was confirmed. Saponarin was quantitatively analyzed in BSE using HPLC, and the inhibitory effect on 3T3-L1 pre-adipocyte differentiation into adipocytes was confirmed by Oil Red O staining, TG assay, and Western blotting. In addition, the inhibitory effect of BSE on adipocyte growth was confirmed through glucose uptake and lipolysis of adipocytes. C57/BL/6N mice were induced to obesity with a high-fat diet, and BSE was administered to confirm the effect on an animal model. Weight gain, morphological changes in adipose tissue, changes in the food efficiency ratio, and blood biochemical changes were observed, and an improvement effect on fatty liver was confirmed. As a result, the anti-obesity effect of BSE was confirmed in vitro, and it was confirmed that this effect was also effective in vivo and that it could be helpful in the treatment of obesity-related diseases.

Highlights

As a result of analyzing the saponarin standards and barley sprout extract (BSE) at 280 nm using HPLC, a peak was confirmed at the same retention time as that of saponarin standard solution and BSE. (Figure 1a,b) This confirmed that saponarin was contained in BSE
This study is a basic study of hot-water barley sprout extract (BSE) as a healthy functional food and medicine to help people establish an anti-obesity strategy suitable for them
Saponarin is a multifunctional flavonoid that is mainly found in barley sprouts, and it is known to have anti-obesity effects

Summary

Introduction

The prevalence of obesity is increasing worldwide. From 1980 to 2015, the obese population increased by 80%, and this rapid increase in obesity is threatening human health beyond the problems of one’s appearance [1]. Obesity is known to cause high blood pressure, fatty liver, and various diseases of the digestive system [2,3]. Excessive accumulation of adipose tissue leads to insulin resistance, which leads to type 2 diabetes, suggesting that obese people are at risk of complications from diabetes [4]. Obesity has even been found to be correlated with an increased risk of cancer [5,6,7]. Obesity itself causes stress, and it is known that stress causes eating disorders, sleep deprivation, and endocrine system abnormalities, which can become further causes of obesity [8]

Methods

Results

Conclusion