Abstract
BackgroundFang-Ji-Huang-Qi-Tang (abbreviated as FJHQT), composed by six medicinal herbs including Radix Stephania Tetrandra, Radix Astragali, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Rhizoma Zingiberis and Fructus Ziziphi Jujubae, is a frequently Chinese prescription for treating painful and inflammatory disorders such as rheumatoid arthritis. When Radix Stephania Tetrandra was misused with Aristolochia species, acute or chronic nephropathy caused by aristolochic acid was happened. Thus, the present study was aimed to identify Radix Stephania Tetrandra and performed the pharmacological and toxicological evaluation of FJHQT extract in rodents.MethodsRadix Stephania Tetrandra was identified by macroscopic and microscopic observation, and the content of tetrandrine in FJHQT extract was measured by high performance liquid chromatography. Then, the pharmacological activities of FJHQT extract with respect to clinical use was investigated with acetic acid-induced writhing response, formalin-induced licking response and carrageenan-induced paw edema. Finally, we evaluated the subacute toxicology of FJHQT extract after 28-day repeated oral administration in rats.ResultsRadix Stephania Tetrandra was correctly used in FJHQT extract, and the content of tetrandrine in FJHQT extract was 2.5 mg/g. FJHQT extract produced a pronounced and dose-dependent antinociceptive and anti-inflammatory effects in three above models. FJHQT extract after 28-day repeated administration did not caused any hematological, biochemical and histological change in rats.ConclusionsWe suggest that FJHQT extract is a high safety index Chinese medicine for antinociceptive and anti-inflammatory application when Radix Stephania Tetrandra was correctly used in FJHQT. Its antinociceptive and anti-inflammatory mechanism might be related to peripheral nociceptive pathway such as prostaglandins.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-015-0527-5) contains supplementary material, which is available to authorized users.
Highlights
MethodsRadix Stephania Tetrandra was identified by macroscopic and microscopic observation, and the content of tetrandrine in FJHQT extract was measured by high performance liquid chromatography
Fang-Ji-Huang-Qi-Tang, composed by six medicinal herbs including Radix Stephania Tetrandra, Radix Astragali, Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Rhizoma Zingiberis and Fructus Ziziphi Jujubae, is a frequently Chinese prescription for treating painful and inflammatory disorders such as rheumatoid arthritis
We further quantified the content of tetrandrine in FJHQT extract after the preparation of FJHQT extract with high performance liquid chromatography (HPLC)
Summary
Preparation of plant extract Radix Stephania Tetrandra (TWU-Plantec-FIJHQT-0001), Radix Astragali (TWU-Plantec-FIJHQT-0002), Rhizoma Atractylodis Macrocephalae (TWU-Plantec-FIJHQT-0003), Radix Glycyrrhizae (TWU-Plantec-FIJHQT-0004), Rhizoma Zingiberis (TWU-Plantec-FIJHQT-0005) and Fructus Ziziphi Jujubae (TWU-Plantec-FIJHQT-0006) were purchased from Taiwan market and identified with macroscopic and microscopic method by Professor Lin Y. FJHQT extract (25, 50, 100 mg/kg) were dissolved in sterile distilled water and administered orally 60 minutes prior to the injection of an inducer. Carrageenan-induced paw edema in rats The anti-inflammatory activity was determined in rats by measuring the mean increase in hind paw volume after the subplantar injection of carrageenan [9]. 30, 60, 90, 120, 150, 180, 210, 240 min after carrageenan injection, the average volume of the backpaws of each animal was determined (Vt), after 3 measurements which did not differ from more than 4%. Control group which rats received sterile distilled water, whereas other groups which rats received FJHQT extract at the doses of 0.1, 0.5, and 1.0 g/kg body weight, respectively. When probability (P) was less than 0.05, the difference was considered to be significant
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
