Anti-Mullerian hormone and AMH gene (rs3741664) polymorphism analysis in Iraqi women with polycystic ovary syndrome

Abstract

Introduction and Aim: Polycystic ovarian syndrome (PCOS) is a complex and heterogeneous endocrine disorder, with the underlying pathogenic mechanisms yet unknown. The purpose of this study was to evaluate the hormonal profile of women with PCOS, as well as their Anti-Mullerian hormonal (AMH) levels and the associated AMH gene (rs3741664) polymorphism. Materials and Methods: The current study included 60 polycystic ovary syndrome (PCOs) patients and 30 healthy women. Serum levels of LH, FSH, TSH, Estrogen and AMH were measured. Polymerase chain reaction (PCR) method was used to genotype the AMH gene (rs3741664). The statistical analysis was performed using GrapPad Prism 9. The rs3741664 genotypes and allele frequencies were calculated and the Hardy-Weinberg equilibrium was utilized to examine the outcomes. Results: There was a substantial rise in LH, TSH, Estrogen, and AMH blood levels in PCOS females as compared to the control female group (P value = 0.05). In contrast, the serum level of FSH was not significantly increased at p value = 0.05. Polymorphism rs3741664 was not significantly associated with serum AMH levels in the population study. Conclusion: LH, TSH, Estrogen, and AMH levels are elevated in PCOS women. As a result, these hormones are regarded as indicators for identifying PCOS in Iraqi females. Polymorphism rs3741664 was not significantly associated with serum AMH levels in the population study. AMH level not significantly associated with genotypes of AMH rs3741664 gene.