Abstract
The causes of toxemia of pregnancy are not fully understood. This project examines rat kidneys to determine if a mouse anti‐renin antibody will cross‐react rat renin‐producing cells in the rat. This will allow further study female reproductive tissues in the rat to examine for renin production. Renin is known as hormone produced by the juxtaglomerular cells of the afferent arteriole of the kidney in response low blood pressure. Also, renin is an immediate downstream target of Class I Hox gene regulation, important in embryonic development. In mice, renin is expressed in embryonic renal, adrenal and gonadal arteries, the adrenal glands, and tissue associated with embryonic pronephric and mesonephric kidneys. Using a renin/GFP reporter in transgenic mice, renin is expressed in placental giant trophoblast cells and uterine/ovarian artery cells of the tunica adventitia. In RCS rats, renin antibody stain that used anti‐rat renin and peroxidase labeled placental giant trophoblast cells and cells of the tunica adventitia of the uterine/ovarian artery. This project examines rat kidney tissue labeled by indirect immuno‐fluorescence with anti‐mouse renin antibody and fluorescent conjugate. Cells in the juxtaglomerular region of the kidney were renin positive. This demonstrates that anti‐mouse renin antibody can dependably label regions in the rat kidney that are known for renin production. This will allow for further studies to determine renin location in rat female reproductive tissues. Dr. Craig Jones of Roswell Park Cancer Institute generously donated mouse renin antibody and lab space for stain procedures. D'Youville College Faculty Council and Department of Math & Natural Sciences supported work.
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