Anti-microbial property of Trigonella foenum-graecum L. Methanol Extract [TFGME] on Pathogenic organisms

Abstract

Methanol extract of Trigonella foenum-graecum L. and its role on pathogenic micro-organisms is the scope of this study. Initially, Trigonella foenum-graecum L. was treated with methanol and the obtained extract was termed as Trigonella foenum-graecum L. Methanol Extract [TFGME]. TFGME shows the presence of Polyphenols, glycosides, phytosterol, saponins and etc., when it subjected to initial preliminary screening. HPLC analysis of TFGME suggests that the presence of several organic molecules as it elutes 9 major peaks after the solvent peak in reverse-phase HPLC at 216nm. In addition, TFGME exhibit anti-microbial property by exhibiting zone of inhibition when it was incubated with pathogenic organisms such as E. coli, S. aureua, Pseudomonas, Salmonela and shigella. The obtained results were recorded in MIC (Minimum Inhibition Concentration) of TFGME values. Furthermore, when TFGME was subjected to analysis of minerals content by using ICP-OES instrument, it was confirmed that TFGME contains aluminium, copper, iron, manganese and zinc in the extract.