Abstract
ABSTRACTMacrophages play crucial roles in combatting infectious diseases by promoting inflammation and phagocytosis. The decline of macrophage phagocytic function will bring many serious consequences, including weakened pathogen clearance. As an avian antibody, immunoglobulin Y (IgY) has been widely used in preventing and treating infectious diseases, but whether it can enhance the phagocytic ability of mammalian macrophages in order to clear pathogens is still unknown. In this study, mouse peritoneal macrophages and THP-1 cells were cultured with anti-lipopolysaccharide (LPS) IgY in vivo or in vitro, respectively. Morphological observation, ELISA, fluorescence immunoassays and flow cytometry were used to study whether IgY could enhance phagocytosis of mammalian macrophages. It was found that without anti-LPS IgY, mouse peritoneal macrophages showed adherent growth with no differentiation and little pseudopod extension; but with anti-LPS IgY, peritoneal macrophages presented more significant characteristics in adherent growth, extension deformation and protruding pseudopods. With flow cytometry, the macrophages from mice injected with anti-LPS IgY exhibited a significantly higher percentage of phagocytosis and index (90.83±2.59% and 4.45±0.13 respectively) compared with phosphate buffered saline (PBS) groups (64.32±1.5%, and 2.36±0.11) and non-immunized groups (65.94%±1.4%, and 2.4±0.15). With phorbol-12-myristate-13-acetate (PMA)-induced THP-1 cells, similar results were found; the percentage and index were significantly higher, with larger body and more pseudopods, for THP-1 cells that were co-incubated with anti-LPS IgY (79.83±0.38% and 2.64±0.03), compared with cells that were co-incubated with PBS (68.07±0.52%, and 1.88±0.03) or non-immunized IgY (74.89±1.14% and 2.30±0.02). The results showed that anti-LPS IgY was effective in promoting the growth of macrophages, pseudopod extension and stronger phagocytic capacity. Our study indicated that anti-LPS IgY could enhance the phagocytic capacity of mammalian macrophages to internalize pathogens more effectively with larger body and more pseudopods. This may be important to encourage IgY to be used to prevent and treat infectious diseases.
Highlights
Phagocytosis is a critical weapon of phagocytic cells; it helps them to hunt and engulf a variety of pathogens and foreign particles (Gray and Botelho, 2017)
We used LPS as the immunogen, and when we prepared the anti-LPS immunoglobulin Y (IgY), we found that the anti-LPS IgY may be a possible tool for the prevention and treatment of LPS injuries (Ma and Zhang, 2010)
THP-1 cells, which are capable of macrophage phagocytosis and secretion of inflammatory mediators, are often used to study the function of human macrophages
Summary
Phagocytosis is a critical weapon of phagocytic cells; it helps them to hunt and engulf a variety of pathogens and foreign particles (Gray and Botelho, 2017). Macrophage, a kind of human phagocytic cell, with the effects of phagocytosis, immune information transmission, cooperation and phagocytosis of antigen processing, plays an important role in the outcome of inflammation (Guillemin and Brew, 2004). When the macrophages are activated, the phagocytic function of the macrophage is significantly enhanced, so as to assist the body in effectively removing harmful components. Previous studies on the host defense function of human Cystic Fibrosis (CF) demonstrated that phagocytosis was significantly reduced in pulmonary phagocytes from pediatric CF lungs (Alexis et al, 2006). IgY may have a positive role in preventing and controlling infectious diseases by enhancing the phagocytic function of mammalian macrophages to remove pathogenic bacteria effectively. This study will mainly focus on whether anti-LPS IgY could enhance the phagocytosis of mammalian macrophages
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
