Abstract
ABSTRACT The effect of ethyl acetate extract of bitter apple, Citrullus colocynthis fruit was studied against S. litura using poison food bioassay experiments. Extraction of toxic compounds from the fruits of C. colocynthis with Soxhlet and purification of extracts with column chromatography were done. In this study, there were seven treatments – five elutions, control and absolute control replicated thrice. The results showed that among the five elutions, ethyl acetate elution 3 had registered 80.00 and 83.33 per cent adult malformation, followed by ethyl acetate elution 1, 2 and 4 compared to control and absolute control in preliminary and confirmation screenings, respectively. The determination of promising dose exhibited that, ethyl acetate elution 3 exhibited the LC50value of 29.62 per cent concentration. In ethyl acetate elutions, it was found that among the elutions, the lowest mean faecal and pupal weight were registered in ethyl acetate elution 3 compared to control and absolute control. Similar trend of results were also observed in confirmation and dose determination experiments.
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