Anti-Inflammatory Effects of Sulfated Polysaccharide from Sargassum Swartzii in Macrophages via Blocking TLR/NF-Κb Signal Transduction.

Thilina U Jayawardena,Hyo-Geun Lee,D P Nagahawatta,Yu-An Lu,You-Jin Jeon,K K Asanka Sanjeewa,D T U Abeytunga,C M Nanayakkara,A P J P Vaas,Dae-Sung Lee

doi:10.3390/md18120601

Abstract

This study involves enzymatic extraction of fucoidan from Sargassum swartzii and further purification via ion-exchange chromatography. The chemical and molecular characteristics of isolated fucoidan is evaluated concerning its anti-inflammatory potential in RAW 264.7 macrophages under LPS induced conditions. Structural properties of fucoidan were assessed via FTIR and NMR spectroscopy. NO production stimulated by LPS was significantly declined by fucoidan. This was witnessed to be achieved via fucoidan acting on mediators such as iNOS and COX-2 including pro-inflammatory cytokines (TNF-α, IL-6, and IL-1β), with dose dependent down-regulation. Further, the effect is exhibited by the suppression of TLR mediated MyD88, IKK complex, ultimately hindering NF-κB and MAPK activation, proposing its therapeutic applications in inflammation related disorders. The research findings provide an insight in relation to the sustainable utilization of fucoidan from marine brown algae S. swartzii as a potent anti-inflammatory agent in the nutritional, pharmaceutical, and cosmeceutical sectors.

Highlights

Brown algae is a promising source of secondary metabolites with potent bioactive properties.Asian countries are renowned for the traditional usage of marine algae as medicinal ingredients.The high availability, active properties, and the low processing cost has intensified the light of attention towards seaweeds in the functional food industry [1]
S. swartzii which was sampled from the coastal area of Hikkaduwa, Sri Lanka
The purification procedure implemented in the study resulted in the isolation of fucoidan fraction (F4) which had well aligned characteristics with commercial fucoidan

Summary

Introduction

Brown algae is a promising source of secondary metabolites with potent bioactive properties.Asian countries are renowned for the traditional usage of marine algae as medicinal ingredients.The high availability, active properties, and the low processing cost has intensified the light of attention towards seaweeds in the functional food industry [1]. Brown algae is a promising source of secondary metabolites with potent bioactive properties. Asian countries are renowned for the traditional usage of marine algae as medicinal ingredients. The high availability, active properties, and the low processing cost has intensified the light of attention towards seaweeds in the functional food industry [1]. Sargassum belongs to the class phaeophyceae and is well exploited for its medicinal aspects [2,3,4], further elaborating studies were continued to the biosynthesis of nanoparticles utilizing extracted polysaccharides [5]. Marine brown algae is an important source of sulfated polysaccharides. Considering its health benefits, marine polysaccharides are well known and candidates in the medicinal, nutraceutical and pharmaceutical sectors. Fucoidan is a sulfate containing polysaccharide and possesses complex formation with valuable biological properties and is popular among Asian food culture.

Methods

Discussion

Conclusion