Abstract
BackgroundKHU14, an ethanolic extract of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) was tested for its anti-inflammatory effects.MethodsThree out of 20 herbs were found to have anti-inflammatory effects. The formulation of these herbs, i.e. KHU14 was tested for croton oil-induced ear edema, carrageenan-induced paw edema, acetic acid-induced capillary permeability, cotton pellet and delayed type hypersensitivity.ResultsKHU14 exhibited anti-inflammatory effects in animal models of acute and chronic inflammation. The anti-inflammatory activity of KHU14 observed was comparable to that of celecoxib. KHU14 inhibited the production of NO and PGE2 in LPS/IFN-gamma-stimulated peritoneal macrophages, and reduced edema and the amount of infiltrated cells in animal models.ConclusionKHU14 exhibited anti-inflammatory effects as demonstrated in typical immunological tests for anti-inflammation in vitro and in vivo.
Highlights
KHU14, an ethanolic extract of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) was tested for its antiinflammatory effects
ELISA kits for interleukin2 and interferon-γ and the immunoassay kit for prostaglandine E2 (PGE2) were purchased from R&D Systems (USA)
Selection of three herbal extracts from 20 herbs To develop potent multiple herbal extracts with antiinflammatory effects, we evaluated the in vitro antiinflammatory effects of each herbal extract in terms of the production of nitric oxide (NO) in LPS and IFN-γ-stimulated peritoneal macrophages (Table 1)
Summary
KHU14, an ethanolic extract of Radix Gentianae Macrophyllae (Qinjiao), Rhizoma Coptidis (Huanglian) and Citri Unshiu Pericarpium (Wenzhou migan) was tested for its antiinflammatory effects. Namely Radix Gentianae Macrophyllae (Qinjiao) [4,5], Rhizoma Coptidis (Huanglian) [6] and Citri Unshiu Pericarpium (Wenzhou migan) [7,8], demonstrated anti-inflammatory effects in various experimental models. Hesperidin [13] and nobiletin [14], both of which exhibit anti-inflammatory effects, are the active ingredients in Citri Unshiu Pericarpium [15,16]. Our in vitro screening and other available information suggests that these three herbs have potential anti-inflammatory effects. These three herbs were selected for a formulation, i.e. KHU14. The present study tests the anti-inflammatory actions of KHU14 in several animal models of inflammation
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