Abstract
Probiotic pretreatment is an effective non-antibiotic strategy for preventing or controlling Salmonella infections. We found that Lactobacillus johnsonii L531, isolated from the colon of a clinically healthy weaned piglet, effectively prevented infection with Salmonella enterica serovar Infantis in a pig model. Newly weaned piglets were intragastrically administered Lactobacillus johnsonii L531 at 1.0 × 1010 CFU/day for 1 week before S. Infantis challenge. Pretreatment with L. johnsonii L531 lessened the severity of diarrhea and ileal inflammation in S. Infantis–infected piglets. Lactobacilli were more abundant in the ileum than jejunum after L. johnsonii L531 pretreatment. Treatment with L. johnsonii L531 reduced the abundance of total bacteria in the ileal mucosa and the production of lipocalin 2 in the jejunum of piglets challenged with Salmonella. Both intestinal morphology and transmission electron microscopy results indicated that L. johnsonii L531 alleviated intestinal tissue damage following S. Infantis challenge, especially in the villus and endoplasmic reticulum (ER). ER stress induced by S. Infantis was attenuated by L. johnsonii L531 treatment. The number of CD4− CCR6+ T cells decreased following S. Infantis challenge, but the percentage of CCR6− IFNγ+ T cells in peripheral blood increased. In intestinal mesenteric lymph nodes, S. Infantis increased the proportion of CCR6+ IFNγ+ T cells, whereas L. johnsonii L531 induced an increase in the proportion of CD4+ CCR6+ T cells in response to S. Infantis infection. Our data thus suggest that L. johnsonii L531 contributes to the maintenance of intestinal homeostasis by modulating T-cell responses and ER stress.
Highlights
Salmonella enterica serovar Infantis, a zoonotic, nontyphoidal S. enterica serovar, is a significant cause of diarrhea and gastroenteritis in humans [1]
Infantis Administration of L. johnsonii L531 reduced the incidence of postweaning diarrhea compared with untreated CN piglets (P = 0.014; Additional file 2)
Salmonella Infantis infection decreased the percentage of CD4− CCR6+ T cells but increased the percentage of CCR6− IFNγ+ T cells To examine the role of CCR6+ T cells in reponse to Salmonella infection, we investigated the percentage of CD4+/− CCR6+/− T cells in peripheral blood at 0, 24, and 192 h following S
Summary
Salmonella enterica serovar Infantis, a zoonotic, nontyphoidal S. enterica serovar, is a significant cause of diarrhea and gastroenteritis in humans [1]. Infantis in pigs with diarrhea [2, 3]. A recent study reported a prevalence of S. Infantis in pigs with Salmonella-associated diarrhea in China of 3.85%. Diarrhea, is associated with dysbiosis [4]. Gutmicrobiota dysbiosis is more likely to induce intestinal inflammation, which could in turn further exacerbate microbiota dysbiosis
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
