Abstract
We conducted a preliminary investigation of the effects of visible light irradiation on plant extracts, and we observed a strong suppressive effect on interleukin (IL) 2 expression with the inhibition of c-Jun amino-terminal kinase (JNK) phosphorylation in Jurkat cells by visible light irradiation to ethanol extract from green soybeans (LIEGS). This effect was produced only by extracts from green soybeans (Glycine max) and not other-color soybeans. LIEGS suppressed the lipopolysaccharide-induced IL-6, IL-12 and TNF-α expression levels in human monocyte THP-1 cells in a concentration-dependent manner. LIEGS was applied for 8 weeks to NC/Nga mice. LIEGS suppressed the development of atopic dermatitis (AD)-like skin lesions and reduced the dermatitis scores of the mice. The light irradiation changed the various types of small-molecule compounds in extracts. Visible light irradiation to daidzein with chlorophyll b induced a novel oxidative product of daidzein. This product suppressed IL-2 expression in Jurkat cells.
Highlights
We conducted a preliminary investigation of the effects of visible light irradiation on plant extracts, and we observed a strong suppressive effect on interleukin (IL) 2 expression with the inhibition of c-Jun amino-terminal kinase (JNK) phosphorylation in Jurkat cells by visible light irradiation to ethanol extract from green soybeans (LIEGS)
We measured the calcium ionophore and phorbol ester-induced IL-2 levels by enzyme-linked immunosorbent assay (ELISA) in the supernatant of Jurkat cell culture with 50 mg/mL of the soybean extracts that were irradiated with visible light at varying distances from a fluorescent lamp to the extract at different times
Twenty-nine cultivated varieties of mature soybean seeds were extracted with ethanol, and the extracts were irradiated by visible light at 70 kilolux for 48 h
Summary
We conducted a preliminary investigation of the effects of visible light irradiation on plant extracts, and we observed a strong suppressive effect on interleukin (IL) 2 expression with the inhibition of c-Jun amino-terminal kinase (JNK) phosphorylation in Jurkat cells by visible light irradiation to ethanol extract from green soybeans (LIEGS). This effect was produced only by extracts from green soybeans (Glycine max) and not other-color soybeans. As an index of the effect, we used IL-2 suppression in Jurkat cells (a human T-cell-like cell line), and we confirmed a strong suppressive effect of visible light irradiation to green soybean ethanol extract on IL-2 expression in Jurkat cells. We recently clarified that water extracts from green soybeans have inhibitory activity for pollinosis with regard to the production of IgE, as well as the ability to regulate B-cell apoptosis, probably by suppressing the expressions of BAFF and APRIL14
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