Abstract
Potentilla erecta (PE) is a small herbaceous plant with four yellow petals belonging to the Rosaceae family. The rhizome of PE has traditionally been used as an antidiarrheal, hemostatic and antihemorrhoidal remedy. PE contains up to 20% tannins and 5% ellagitannins, mainly agrimoniin. Agrimoniin is a hydrolyzable tannin that is a potent radical scavenger. In this study we tested the anti-inflammatory effect of four PE fractions with increasing amounts of agrimoniin obtained by Sephadex column separation. First, we analyzed in HaCaT keratinocytes the expression of cyclooxygenase-2 (COX-2) induced by ultraviolet-B (UVB) irradiation. As COX-2 catalyzes the metabolism of arachidonic acid to prostanoids such as PGE2, we also measured the PGE2 concentration in cell culture supernatants. PE inhibited UVB-induced COX-2 expression in HaCaT cells and dose-dependently reduced PGE2. The PE fraction with the highest agrimoniin amount (PE4) was the most effective in this experiment, whereas fraction PE1 containing mainly sugars had no effect. PE4 also dose dependently inhibited the phosphorylation of the epidermal growth factor receptor (EGFR) which plays a crucial role in UVB-mediated COX-2 upregulation. A placebo-controlled UV-erythema study with increasing concentrations of PE4 demonstrated a dose dependent inhibition of UVB-induced inflammation in vivo. Similarly, PE4 significantly reduced UVB-induced PGE2 production in suction blister fluid in vivo. In summary, PE fractions with a high agrimoniin content display anti-inflammatory effects in vitro and in vivo in models of UVB-induced inflammation.
Highlights
Potentilla erecta (PE) is a small plant with yellow petals that belongs to the family of Rosaceae.The rhizome of PE contains up to 20% tannins [1]
In the present study we tested the anti-inflammatory effect of crude PE extract as well as 4 PE fractions with increasing agrimoniin concentration
Models of UVB induced inflammation in vitro revealed that already the crude PE extract has an anti-inflammatory effect as shown by inhibition of UVB- induced COX-2 expression and PGE2 -production
Summary
Potentilla erecta (PE) is a small plant with yellow petals that belongs to the family of Rosaceae. Tannins are grouped into hydrolyzable tannins, consisting of gallic acid and/or ellagic acid and in temperate, arctic and alpine zones of the Northern hemisphere [1] Because of their aromatic ring sugarstructures moieties, condensed or proanthocyanidins, composed of flavanols, its and tannins can act as tannins. Agrimoniin was about 20 times more effective than EGCG, the main tannin of green tea, and comparable that agrimoniin (or an agrimoniin-rich acetonic PE fraction) had outstanding capacities as an to the best anti-allergic drug Azelastine. Vennat, Bos and colleagues found that agrimoniin From these results it seemed promising to further an agrimoniin-rich acetonic PE fraction) had outstanding capacities as an antioxidant, astringent, investigate the potential use ofFrom.
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