Anti-inflammatory effect of salidroside on phorbol-12-myristate-13-acetate plus A23187-mediated inflammation in HMC-1 cells.

Abstract

Salidroside [2-(4-hydroxyphenyl)ethyl β-D-gluco-pyranoside(SAS)] has been identified as the most potent ingredient of the plant Rhodiola roseaL. Previous studies have demonstrated that it possesses a number of pharmacological properties, including anti-aging, anti-fatigue, antioxidant, anticancer and anti-inflammatory properties. In this study, to ascertain the molecular mechanisms responsible for the anti-inflammatory activity of SAS, we used phorbol-12-myristate-13-acetate(PMA) plus A23187 to induce inflammation in human mast cell line-1(HMC-1). The HMC-1 cells were treated with SAS prior to being stimulated with PMA plus A23187. Pro-inflammatory cytokine production was measured by enzyme-linked immunosorbent assay(ELISA) and reverse transcription-polymerase chain reaction(RT-PCR). Western blot analysis was used to examine the activation of mitogen-activated protein kinases(MAPKs) and nuclear factor κ-light-chain-enhancer of activated Bcells(NF-κB). SAS inhibited the mRNA expression and production of interleukin(IL)-6, IL-8 and tumor necrosis factor(TNF). In cells stimulated with PMA plus A23187, SAS suppressed the phosphorylation of extracellular signal-regulated kinase(ERK)1/2 and c-jun N-terminal kinase1/2(JNK1/2), but not that of p38MAPK. SAS suppressed the expression of NF-κB in the nucleus. On the whole, our results suggest that SAS exerts an anti-inflammatory effect by inhibiting the production of pro-inflammatory cytokines through the blocking of the NF-κB and MAPK signaling pathways.