Anti-inflammatory effect of epidermal growth factor conjugated silk fibroin immobilized polyurethane ameliorates diabetic burn wound healing

Abstract

Few studies are reported on immunomodulatory potential of non-mulberry (Antheraea mylitta) silk fibroin (SF) combined with polyurethane (PU) in diabetic wound healing. In this study, PU/SF (Antheraea mylitta) scaffolds were fabricated by blending and immobilization techniques. Effective SF dosage was determined and incorporated according to minimum inhibitory concentrations against wound associated bacterial strains while fabricating scaffold. Dermal fibroblast NIH3T3 cells were seeded on epidermal growth factor (EGF) treated and untreated PU/SF scaffolds, fabricated by blending and immobilization techniques. Fibroblast seeded PU/SF scaffolds were investigated for anti-inflammatory response in wound recovering potential comparing with Acticoat™ in third degree burn of streptozotocin induced diabetic rats. At 16th, 24th days, promising healing was achieved with faster granulation, enhanced collagenization, patterned re-epithelialization by EGF treated cellular immobilized PU/SF in normal, hyperglycemic burn. Biomarkers of different healing stages, CD31 (haemostasis), Ki67 (proliferative), alpha-sma, COL III (maturation) were examined. Since hyperglycemic burn is characterized by inflated pro-inflammatory cytokines, serum, tissue IL-6,8,10 were recorded, which revealed timely restoration of inflated IL-6,8 and protection against IL-10 elevation by cellular immobilized PU/SF compared to Acticoat™ (p ≤ 0.05), control (p ≤ 0.01). E-cadherin (gap junction protein), MMP 9 response suggested anti-inflammatory role of PU/SF on accelerated healing of thermal injury as potent dermal substitute.