Anti-inflammatory effect of a novel millet gliadin peptide on mice with colitis

Abstract

A novel peptide (VSAAAA) was obtained after the isolation, purification, and identification of millet gliadin. In order to explore the anti-inflammatory effect of peptide VSAAAA in vivo, the peptide VSAAAA was administered in the first three weeks, and ulcerative colitis was induced in mice with DSS aqueous solution in the last week. Results showed that the disease activity index and histopathological changes in the colon were significantly reduced after VSAAAAA intervention. The expression levels of serum proinflammatory cytokines in mice were significantly reduced. In addition, VSAAAA gavage significantly alleviated decrease in the expression levels of the intestinal nexus proteins ZO-1 and occludin and increase in the expression levels of p65 and p-p65 proteins in the NF-κB signaling pathway. Moreover, the intestinal flora composition of colitis mice was regulated by decreasing the relative abundance of harmful bacteria (Firmicutes) and increasing the relative abundant of beneficial bacteria (Bacteroides).