Abstract
In the present work we determined phenolic and flavonoids content of Eriobotrya japonica leaves extracts and fractions and their antioxidant and anti-inflammatory properties. To evaluate the inhibition of inflammatory PLA2 and antioxidant effects of extracts and fractions from Eriobotrya japonica leaves. Antioxidant activity was evaluated with DPPH radical scavenging assay and anti-inflammatory effect of fractions was measured by their inhibition potency on the human pro-inflammatory phospholipase A2 (group IIA). The EtOH/EtOAc 2:1 extract exhibited a potent inhibition of the hG-IIA with an IC50 values of 8 µg/ml. It also shows an antioxidant activity measured on DPPH with an IC50 of 42 µg/ml. Fractionation shows that CH2Cl2/MeOH 0:1 fraction was the rich one on flavonoids compounds (4.3 mg/g dry weight) and demonstrates a high antioxidant activity with an IC50 of 12 µg/ml. The anti-inflammatory evaluation demonstrates that the same fraction was the best one to inhibit the pro-inflammatory phospholipase A2 group IIA with an IC50 of 4 µg/ml. Study conducted on Eriobotrya japonica shows that CH2Cl2/MeOH 0:1 fraction inhibits efficiently the hG-IIA phospholipase. which is considered as pro-inflammatory enzyme.
Highlights
Eriobotrya japonica Lindl, known as ‘loquat’, belongs to the Rosaceae family
African Health Sciences Vol 15 Issue 2, June 2015 in inflammatory process[17,18,19] and many phospholipases A2 inhibitors have been discovered and their effectiveness have been proved as a treatment of inflammatory diseases[20,21,22]. Because overproduction of these inflammatory mediators might cause inflammatory damage, we focused in the present study on the evaluation of the anti-inflammatory effect of Leaves of Eriobotrya japonica (LEJ) extracts by measuring the inhibition of the pro-inflammatory secreted phospholipase A2 (sPLA2) group IIA as well as their antioxidant activity
Results show that the EtOH/EtOAc 2:1 extract inhibits the human inflammatory phospholipase A2 group IIA (hG-IIA) PLA2 and the phospholipase A2 group IB (pG-IB) with an inhibitory concentration at 50 % (IC50) of 8 μg/mL and 1200 μg/mL, respectively (Table 3). This finding proves that this extract inhibits preferentially hG-IIA with a relative specificity inhibition factor of about 150. Fractions from this extract were tested for their ability to inhibit these two PLA2 and results revealed that CH2Cl2/MeOH 0:1 fraction is the most interesting one (Table 3)
Summary
Eriobotrya japonica Lindl, known as ‘loquat’, belongs to the Rosaceae family This plant is an evergreen shrub or small tree with narrow leaves that are dark green on the upper surface and have a lighter color under surface. LEJ has anti-inflammatory activity in a 12-O-tetradecanoylphorbol-13-acetate induced inflammation model. Results: The EtOH/EtOAc 2:1 extract exhibited a potent inhibition of the hG-IIA with an IC50 values of 8 μg/ml It shows an antioxidant activity measured on DPPH with an IC50 of 42 μg/ml. The anti-inflammatory evaluation demonstrates that the same fraction was the best one to inhibit the pro-inflammatory phospholipase A2 group IIA with an IC50 of 4 μg/ml. Conclusion: Study conducted on Eriobotrya japonica shows that CH2Cl2/MeOH 0:1 fraction inhibits efficiently the hGIIA phospholipase.which is considered as pro-inflammatory enzyme
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