Anti-inflammatory and antioxidant effects of zinc and vitamin D on nicotine-induced oxidative stress in adult male rats

Abstract

Current study aimed to investigation the protective effects of zinc and vitamin D in attenuating alveolar response, as anti-inflammatory and antioxidant, in nicotine stressed male rats. Thirty adult males Wistar rats, as a model of mammals, randomly assigned to five equal groups and treatment as follows for 14 days: Control (C) drenched vehicle without treatment, G1 = injected nicotine 1.5 mg/ kg b.w. i/P, G2 administrated orally of zinc 60 mg/ kg b.w., G3 = administrated orally of vitamin D 250 µg/ kg b.w. and G4 administrated orally both of zinc and vitamin D with same doses and stressed by nicotine 1.5 mg/ kg b.w. i/P. At the end of the experiment, rats were anesthetized blood samples have been collected, and then iron, cytokines (IL-6) and malondialdehyde (MDA) and glutathione peroxidase (GPX) were estimation in in serum and lung tissues. The result showed significant (p ≤ 0.5) increase in serum MDA in group G1 when compared with other groups. While significant (p ≤ 0.5) increase in serum GPx in all treatment group in comparison with nicotine treated group and control.