Anti-inflammatory and antinociceptive features of Bryonia alba L.: As a possible alternative in treating rheumatism

Mert Ilhan,Ibrahim Tümen,Esra Küpeli Akkol,Fatma Tuğçe Gürağaç Dereli

doi:10.1515/chem-2019-0003

Abstract

AbstractBryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. B. alba roots were extracted with n-hexane, ethyl acetate (EtOAc), and methanol, successively. The carrageenan-induced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. DPPH-radical-scavenging activity, ABTS radical–scavenging activity, total antioxidant activity, and hydroxyl radical– scavenging activity were assessed. Furthermore, the total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. Moreover, it had high phenolic and flavonoid contents. Therefore, B. alba roots could be alternative treatment method for rheumatic disorders.

Highlights

Bryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain
The present study was performed to evaluate the anti-inflammatory, antinociceptive, and antioxidant activities of n-hexane, ethyl acetate, and methanol extracts prepared from B. alba roots
The paw edema induced by carrageenan has been used widely for assessing the anti-inflammatory action of steroidal and nonsteroidal drugs containing numerous chemical mediators, including serotonin, bradykinin, Table 1: Effects of test materials on carrageenan-induced hind paw edema in mice

Summary

Introduction

Abstract: Bryonia species have traditionally been used as a diuretic and laxative, to reduce edema and relieve joint pain. B. alba roots are used especially to treat rheumatic pain and applied to painful joints in Turkish folk medicine. The carrageenaninduced hind paw edema model and the Whittle method were used to evaluate the anti-inflammatory activity of the extracts in mice. The p-benzoquinone-induced abdominal constriction test and tail flick test were used to evaluate the antinociceptive activity of the extracts in mice. The total phenolic and total flavonoid contents were calculated for all the extracts. The EtOAc extract prepared from B. alba roots had the most promising anti-inflammatory, antinociceptive, and antioxidant activities. It had high phenolic and flavonoid contents. B. alba roots could be alternative treatment method for rheumatic disorders

Objectives

Methods

Results