Anti-inflammatory activity of Myristica fragrans extract

Abstract

In simple terms, inflammation can be defined as a reaction from the body to an injury in living tissue. Anti-inflammatory drugs help in controlling and reducing this inflammation. Natural spices showing anti-inflammatory properties with no side effects, hence they can be used as an efficient anti-inflammatory drug in the near future. To determine the anti-inflammatory activity of Myristica fragrans (Nutmeg) using MTT Assay. The plant material was obtained as a gift sample from Life Care Phytolabs Private Limited. An extract was prepared from the sample. Cell viability assay – MTT Assay was performed, and Raw cell line 247 was used to study the anti-inflammatory potential of the extract. The results collected were put into a graph and table for discussion. A gradual decrease in the number of inflammatory cells as the concentration of the extract was increased was observed in the inflammatory cell line. The cell viability, which was 7.08% when the concentration of the extract was 1ng increased up to 30.6% when the concentration of the extract was increased up to 100ug. The MTT assay test on a raw cell line 247 showed that the Myristica fragrans extract exhibits some level of the anti-inflammatory property. Further research on isolating the specific component of the extract responsible for its anti-inflammatory property can be done in the future.