Anti-Inflammatory Activity of Mangosteen (Garcinia Mangostana Linn.) Rind Extract Nanoemulgel and Gel Dosage Forms

Abstract

Mangosteen (Garcinia mangostana Linn.) rind is known for its anti-inflammatory activity. Inflammation of local tissue can be overcome by topical administration of dosage forms. In an effort to improve the quality of topical drug delivery, nanoparticle technology can be an option.The purpose of this study is to determine the activity of gel and nanoemulgel dosage forms containing fractions of mangosteen rind extract (n-hexane: ethyl acetate). The gel dosage form of mangosteen rind fractions was successfully prepared. Its physical and chemical properties were evaluated, and the results were within the expected range. The spreadibility of the formulations was between 5-7cm and the pH was between 4.5 and 6.5.The 0.0625% and 0.125% mangosteen rind fraction concentrations are the formulas by which nanoemulgel was successfully formed, resulting in non-separating phases, percent transmittance of 96.997 ± 0.137% and 94.253 ± 0.134% respectively, particle size of 17.437 ± 0.427 and 17.240 ± 0.276 nm; potential zeta of 5.183 ± 0.202 and -10.143 ± 0.238. In the inflammatory test of carrageenan induced laboratory mice, nanoemulgel containing 0.0625% and 0.125% mangosteen rind fraction concentrations produced better percent inhibition (p<0.05) compared to gel containing 0.1%, 0.5%, and 1% mangosteen rind fraction concentrationsin the 90th minute, but the difference was not significant in the 120thminute through the end of thetest. The nanoemulgel containing 0.0625% and 0.125% mangosteen rind fraction concentrations have an unsignificant difference in results (p>0.05) when compared to the reference drug (diclofenac sodium) in the 90th minute.