Anti-Inflammatory Activity of Cream Type O/W with Concentration Variation of Essential Oils of Clove (Syzigium aromaticum)

Abstract

Clove essential oil (Syzigium aromaticum) has been proven as anti-inflammatory agent. Formulation in the cream type O/W preparations was required to be utilized by the community. The aims of this research was to determined activity of cream as anti-inflammatory with concentration variation of essential oil. Cream was made by melting method with concentration variation of clove essential oil: 2.5%; 5%; 10%. The cream was evaluated for anti-inflammatory activities by using animal test BALB/c strain of mice that previously has been induced inflammation with crotton oil. The results showed that increasing concentrations of clove essential oils tended to increase the thickness of skin folds, thickness of the epidermis, the number of inflammatory cells and the number of cells with COX-2 expression. The optimal concentration of essential oils in cream type O/W as anti-inflammatory was 2.5%. Keywords: Clove Essential Oil, Cream Type O/W, Anti-Inflammatory.