Anti-Inflammatory Activities of an Extract of In Vitro Grown Adventitious Shoots of Toona sinensis in LPS-Treated RAW264.7 and Propionibacterium acnes-Treated HaCaT Cells

Hyeon-Ji Lim,In-Sun Park,Sang-Jun Kim,Seung-Il Jeong,Eun Yee Jie,Suk Weon Kim,Chan-Hun Jung,Kang-Yeol Yu,Woo Seok Ahn

doi:10.3390/plants9121701

Hyeon-Ji Lim, In-Sun Park + Show 7 more

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https://doi.org/10.3390/plants9121701

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Abstract

Toona sinensis has been traditionally used to treat dysentery, enteritis, flatulence, and itchiness. However, the existence of anti-inflammatory effects of T. sinensis on Propionibacterium acnes-induced skin disease is unknown. In vitro cultures of plant cells and tissues produced under controlled conditions offer a continuous production platform for plant natural products including pigments and anti-inflammatory agents. In this study, we determine the anti-inflammatory activities of an extract of in vitro grown adventitious shoots of T. sinensis on P. acnes, the etiologic agent of skin inflammation. The extract of T. sinensis showed antioxidant and anti-inflammatory activity in LPS-treated RAW264.7 cells. It also had antibacterial activity and anti-inflammatory effects on P. acnes-treated HaCaT cells. In addition, these effects were regulated by suppression of the mitogen-activated protein kinase (MAPK) pathways. These results suggesting the potential application of adventitious shoots of T. sinensis grown with an in vitro proliferation system as a medicine for treating P. acnes-induced inflammatory skin disease.

Highlights

Acne is one of the most common skin diseases globally, affecting approximately 650 million adolescents and adults [1,2]
Adventitious shoots were successfully induced from hypocotyl explants of T. sinensis when they were cultured on MS2B01N medium after four weeks of incubation
These results suggest that T. sinensis (TS) extract can inhibit the production of pro-cytokines

Summary

Introduction

Acne is one of the most common skin diseases globally, affecting approximately 650 million adolescents and adults [1,2]. Acne is a chronic inflammatory disease which causes skin redness, itching, and inflammation [3]. The exact causes of acne remain unclear, environmental factors such as heredity, diet, stress, hormonal imbalance, and a narrow equilibrium between Propioninbacterium acnes (P. acnes) and other skin flora might play important roles in acne onset [4,5,6]. Among these factors, P. acnes, a Gram-positive anaerobic bacterium, has been reported to be a major factor in acne inflammatory reaction [7]. Enhanced expression of Plants 2020, 9, 1701; doi:10.3390/plants9121701 www.mdpi.com/journal/plants

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