Abstract
BackgroundDioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis.MethodsSixty male Wistar rats were divided into six groups: the normal group, model group, colchicine group (COL) and three total saponin groups (RDN) (high dose [160 mg/kg], middle dose [80 mg/kg] and low dose [40 mg/kg]). HE staining was used to detect the histopathologic changes of the synovial tissue of joint. Immunohistochemical method was used to detect the protein expressions of P-38, p-P38, JNK, p-JNK, ERK1/2, p-ERK1/2, MEK1/2, p-MEK1/2, MKK4, p-MKK4, ICAM1, VCAM1, and PPARγ in the synovial tissue of joint. Realtime PCR and WB methods were used to detect the mRNA and protein expressions of PPARγ and AdipoR2 in the synovial tissue of joint. The contents of CXCL1 and ADP in the blood serum were measured by Elisa method.ResultsOur study showed that RDN could improve the situation of the synovial tissue, reduce the protein expressions of MKK4, p-MEK1/2, p-JNK, p-ERK1/2, ICAM1. They could also decrease the content of CXCL1 and increase the content of ADP in the blood serum.ConclusionRDN has good effect of anti-inflammation. This is in part realized by influencing MAPK signalling pathway. It provides a new visual angle to reveal the mechanism of RDN to treat GA.
Highlights
Dioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis
Our results showed that Rhizoma Dioscorea Nipponicae (RDN) reduces MKK4, p-mitogen-activated extracellular signal-regulated kinase1/2 (MEK1/2), p-c-Jun N-terminal kinase (JNK), p-extracellular signal-regulated kinase-1/2 (ERK1/2), and Intercellular adhesion molecular1 (ICAM1) protein expression and increases PPARγ mRNA and protein expression
RDN regulates the abnormal expression of MKK4, pMEK1/2, p-JNK, p-ERK1/2 and ICAM1 protein and PPARγ mRNA and protein in the synovial joint tissue
Summary
Dioscorea nipponica Makino is widely used in traditional Chinese medicine to treat gouty arthritis. Gouty arthritis (GA) has recently been occurring more frequently in China and worldwide [1]. It is common in men compared to women [2]. This disorder results from high serum uric acid concentrations; monosodium urate (MSU) crystals are subsequently formed and deposited in both joints and tissues. It is urgent to identify new drugs to treat GA since it was detrimental to the health of the patients and especially in the condition of comorbidities [6]. Mitogen-activated protein kinases (MAPKs) play an important role during the pathogenesis of GA [7]. MAPKs are classified into main three types: extracellular signal-regulated kinase-1/2 (ERK1/2), c-Jun N-terminal
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