Anti-inflammation effects of hydrogen saline in LPS activated macrophages and carrageenan induced paw oedema

Zheng Xu,Zhen Zhu,Jiangrui Zhou,Chunlei Jiang,Jianmei Cai,Xuejun Sun

doi:10.1186/1476-9255-9-2

Abstract

BackgroundOxidative stress is thought to play an important role in the pathogenesis of inflammation. Recent studies have found that hydrogen gas has the effect of eliminating free radicals. Whether hydrogen saline (more convenient to be used than hydrogen gas) has the anti-inflammation effect or not is still unknown.MethodsCarrageenan-induced paw oedema and LPS-activated macrophages are studied in this article. Injection of carrageenan into the foot of a mouse elicited an acute inflammatory response characterized by increase of foot volume and infiltration of neutrophils. While tumor necrosis factorα(TNF-α) secreted by activated macrophages was determined by ELISA and real-time PCR.ResultsAll parameters of inflammation (foot volume, infiltration of neutrophils, amount of TNF-α and the level of TNF-α's mRNA) were attenuated by the hydrogen saline treatment.ConclusionAs a more convenient way than inhaling H2, hydrogen saline exhibits a protective effect against inflammation and it might provide a novel therapeutic approach for inflammatory diseases.

Highlights

IntroductionOxidative stress is thought to play an important role in the pathogenesis of inflammation
Oxidative stress is thought to play an important role in the pathogenesis of inflammation through direct injurious effects, and by involvement in molecular mechanisms [1]
The time-dependent curve shows that the paw swelling ratio will rise till 4 hours after carrageenan injection. (Figure 1A) Hydrogen saline showed bell shaped effect of down-regulating carrageenan-induced paw swelling, compared with the vehicle control group, which received an equal volume of vehicle only. (Figure 1A.B)

Summary

Introduction

Oxidative stress is thought to play an important role in the pathogenesis of inflammation. Recent studies have found that hydrogen gas has the effect of eliminating free radicals. Whether hydrogen saline (more convenient to be used than hydrogen gas) has the anti-inflammation effect or not is still unknown We expect to examine the effects of hydrogen saline on inflammation models

Methods

Results

Discussion

Conclusion