Anti-imflammatory and cancer cell inhibitory activities of isolated compounds from Aspidistra triradiata N. Vislobokov

Abstract

Background: The genus Aspidistra possesses biological activities such as antivirus, anti-cancer, anti-inflammation. The study aimed to isolate compounds, evaluate the anti-inflammatory and cytotoxic activities of A. triradiata. Materials and methods: The whole plant of A. triradiata was isolated by chromatographic methods, determined the structure of isolated compounds by NMR spectrum. The anti-inflammatory effect was evaluated by the inhibitory activity against nitric oxide production. The cytotoxic activity was determined by SRB method. Results: Four compounds were isolated from A. triradiata, including (25R)-spirostane-1β,2β,3β,4β,5β,6β-hexol (1), (25R)-17α-hydroxy-spirost-5-en-3β-yl α-L-rhamnopyranosyl-(1→4)-α-L-rhamnopyranosyl-(1→4)-β-D-glucopyranoside (2), 2-methoxy pancracin (3) and 2-methyl-3-(4,5,7-trihydroxy-8-hydroxymethyltetrahydro-6H-4-pyranyl)-2-propenoic acid (4). Compounds 1, 2 and 4 exhibited moderate anti-inflammatory activities with IC50 values in the range of 59.84 - 98.96 µg/mL. Compound 3 exhibited potent cytotoxicity against the LU-1 and KB cancer cell lines with IC50 values in the range of 0.95 - 0.98 µg/mL, compound 1 showed moderate activity with IC50 values ranging from 47.55 to 72.32 µg/mL. Conclusion: The chemical composition of A. triradiata has been elucidated and some isolated compounds possessing potential anti-inflammatory and cytotoxic activities were discovered. Key words: anti-inflammatory, nitric oxide, cancer, Aspidistra triradiata, NMR spectra.