Anti-hyaluronidase and cytotoxic activities of fucoxanthin cis/trans isomers extracted and characterized from 13 brown seaweeds

Abstract

Anti-oxidant, anti-hyaluronidase and anti-cancer activities of fucoxanthin isomers extracted from 13 brown seaweeds were studied by profiling through high-performance thin layer chromatography. The fucoxanthin exhibiting significantly (p < 0.05) high cytotoxicity was characterized using Liquid Chromatography-Mass Spectroscopy (LC-MS). Species belonging to Dictyotales recorded the highest amount of fucoxanthin followed by Fucales and Ectocarpales. Among 13 species, the fucoxanthin of Lobophora variegata exhibits high bioactivity followed by Padina gymnospora, and Sargassum vulgare Pamban. Docking study revealed that fucoxanthin isomers had extremely good inhibitory binding with hyaluronidase by recording negative binding energy (−6.8, −8.2, and −7.8 kcal/mol). Fucoxanthin trans form exhibiting high bioactivity can hold at −20 0C or at room temperature in dark. The LC-MS fragment pattern of highly active fucoxanthin of P. gymnospora was at m/z 660 and 681.9; L. variegata at m/z 581.8, 640, 659, 658.1, and 680; and S. vulgare Pamban at m/z 659.61. Fucoxanthin extracted from the species of Dictyotales exhibits significantly high bioactivity against Michigan Cancer Foundation-7 cell lines with extremely low IC50 value (P. gymnospora 2.02 mL−1; L variegata 1.74 mL−1) followed by S. vulgare Pamban (Fucales) with IC50 value 2.79 g mL−1, that can be taken up for anti-cancer drug and skin care product development.