Abstract
Sera of female mice, immunized with estradiol-albumin conjugates, were tested for restricted heterogeneity of the anti-estradiol antibody response and for the development of antinuclear antibody (ANA). We found sera with the highest estradiol binding capacity gave clear evidence of clonal dominance when analyzed by isoelectric focusing. A sub-set of the immunized animals, approximately one-third, developed positive ANA serologies within 12 weeks of the primary immunization. All positive sera gave homogeneous patterns of staining in an avidin-biotin amplified indirect immunofluorescence assay. Our results point to a spontaneous production of ANA in at least some animals immunized with a ligand which binds to a nuclear receptor.
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