Anti-HIV, antitumor and immunomodulatory activities of paclitaxel from fermentation broth using molecular imprinting technique

Junhyok Ryang,Tzi Bun Ng,Yangyang Song,Fang Liu,Yan Yan

doi:10.1186/s13568-019-0915-1

Junhyok Ryang, Tzi Bun Ng + Show 3 more

Open Access

https://doi.org/10.1186/s13568-019-0915-1

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Journal: AMB Express	Publication Date: Dec 1, 2019
Citations: 14	License type: open-access

Affiliation: Chinese University of Hong Kong, Nankai University

Abstract

In this study, a single component paclitaxel was obtained from fermentation broth by molecular imprinting technique, and its antiviral, antitumor and immunomodulatory activities were studied. The results showed that paclitaxel had a good inhibitory activity on human breast cancer MCF-7 cells and showed a concentration- dependent relationship with an IC50 of about 15 μg/mL in the sulforhodamine B assay. At the same time, paclitaxel exerted a weak inhibitory activity on cervical cancer Hela cells. In addition, paclitaxel not only inhibited the invasion of HIV-1 pseudovirus into cells, but also exhibited inhibitory activity to a certain extent after viral invasion of the cells. At a paclitaxel concentration of 20 μg/mL, the inhibition of HIV-1 pseudovirus reached about 66%. The inhibition of HIV-1 protease activity was concentration-dependent. At a concentration of 20 μg/mL, the inhibitory effect of paclitaxel on HIV-1 protease was similar to that of the positive control pepstatin A, being 15.8%. The HIV-1 integrase inhibiting activity of paclitaxel was relatively weak. Paclitaxel significantly up-regulated the expression of interleukin-6.

Highlights

Among plant-derived natural products, paclitaxel (C47H51NO14), which was first isolated from the bark of the Pacific yew Taxus brevifolia, known commercially as taxol, is a chemotherapeutic diterpenoid drug that exhibits potent anticancer activity (Kasaei et al 2017; Zhou et al 2010).Due to its complex structure, unique medicinal mechanism and good anti-tumor activity, paclitaxel has been the subject of research of many scholars (Lasala et al 2006; Oberlies and Kroll 2004)
Preparation and High‐performance liquid chromatographic (HPLC) analysis of paclitaxel by molecularly imprinted method The presence of paclitaxel in the crude extracts was confirmed by HPLC analysis
Paclitaxel was isolated from the bark of yew (Shankar Naik 2019)

Summary

Introduction

Among plant-derived natural products, paclitaxel (C47H51NO14), which was first isolated from the bark of the Pacific yew Taxus brevifolia, known commercially as taxol, is a chemotherapeutic diterpenoid drug that exhibits potent anticancer activity (Kasaei et al 2017; Zhou et al 2010).Due to its complex structure, unique medicinal mechanism and good anti-tumor activity, paclitaxel has been the subject of research of many scholars (Lasala et al 2006; Oberlies and Kroll 2004). Among plant-derived natural products, paclitaxel (C47H51NO14), which was first isolated from the bark of the Pacific yew Taxus brevifolia, known commercially as taxol, is a chemotherapeutic diterpenoid drug that exhibits potent anticancer activity (Kasaei et al 2017; Zhou et al 2010). The main source of paclitaxel is still dependent on the yew tree. Researchers aim at taxol production by means of several modern techniques including chemical synthesis, semi-synthesis method and plant tissue culture method. These methods have both advantages and disadvantages (Ismaiel et al 2017; Shankar Naik 2019). Developing a cost-effective paclitaxel fermentation process by microorganisms has

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Conclusion