ANTI FUNGAL ACTIVITY OF AQUEOUS LEAF EXTRACT OF OCIMUM SANCTUM ON DOMINANT FUNGAL SPECIES OF MONUMENT WITH SPECIAL REFERENCE TO BHAND DEOL TEMPLE AT ARANG OF CHHATTISGARH

Abstract

ABSTRACT Four common species of fungi isolated A. niger, Rizopous, Cladpsporium and Curvularia Lunata from archaeological site were subjected to laboratory experiment involving in vitro control of the fungal species using plant extracts. Aqueous leaves extract at 10%, 20%, 30% and 40% with the control (basal medium) concentrations tested on potato dextrose agar (PDA) for activity against mycelium growth were determined at 26±1 0 C with three replicated plates. Fungal growth values recorded were generally low compared with the control (without extract petri plate). Inhibitory action of the extract on fungal growth increased with increases in concentration of extract. A study was carried out to evaluate the antifungal properties of Ocimum Sanctum Linn. Aqueous extract on common fungal species, isolated from Bhand Deol temple at Arang of Chhattisgarh state using the well in PDA media. The in vitro studies have been performed by using leaf aqueous extract of Ocimum Sanctum Linn. (Tulsi plant). Extract showed antifungal activity. Different concentration viz. 10 %, 20%, 30% and 40% of solution prepared for the study. It was found that the plant extract at 40% concentration were effective in reducing the mycelial growth of A. niger, Rizopous, cladpsporium sp. whereas 30% of plant extract effective for Curvularia Lunata. Concentrations of extract which inhibit 75% and above having known as effective in this study. Plant extracts readily available and affordable and environmentally friendly in the control of fungal disease.