Abstract
BackgroundHolothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats.MethodsFifty four male Wistar albino rats were assigned into two main groups, the Sham-operated control and bile duct ligated (BDL) group. After 14 days of surgery, the animals of the group I (Sham control) received distilled water only for 7, 14 and 28 days. Second group (BDL group) was divided into 2 subgroups, animals of these subgroups treated for 7, 14 and 28 consecutive days as follow: subgroup I (BDL), rats of this subgroup administered distilled water orally. Subgroup II (HaE), animals of this subgroup treated orally with HaE (200 mg/kg body weight).ResultsThe HaE revealed significant antifibrotic effect as evident by decreasing the levels of total conjugated and unconjugated bilirubin and the activities of serum aminotransferases (ASAT and ALAT) and alkaline phosphatase (ALP) as well as malondialdehyde (MDA) level, and increasing the serum albumin, glutathione reduced (GSH) levels. Treatment with HaE normalized the antioxidant enzyme, glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activities activities.ConclusionThe present prospective study correlated the antifibrotic effect of HaE to its direct antioxidant effect that can be related to its contents of phenolic compounds specially chlorogenic acid, pyrogallol, rutin and coumaric acid.
Highlights
Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast
The bile salts are partly responsible for the plasma membrane damage seen in the bile-duct ligated models that leads to further oxidative stress [14,15]
The present study aims to assess the anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats and to elicit the underlying in vivo anti-oxidative mechanisms
Summary
Holothuria arenicola is the most important and abundant sea cucumber species in the Mediterranean Sea on the Egyptian coast. The present study aims to assess the anti-oxidative and anticholestatic effects of the sea cucumber Holothuria arenicola extract (HaE) in a model of bile duct ligation in male albino rats. Abnormal flux of bile acids and bilirubin in the liver leads to retention and accumulation of toxic hydrophobic bile salts within hepatocytes [4], causing inflammatory reactions, hepatocyte death, and periductular fibrosis [5]. Bile duct ligation (BDL) is a typical model of biliary cholestasis in animals [12], that induced oxidative damage and fibrosis in rats [13]. The bile salts are partly responsible for the plasma membrane damage seen in the bile-duct ligated models that leads to further oxidative stress [14,15]
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