Abstract
Purpose: To study the anti-endometriotic effect of Angelica sinensis (Oliv.) Diels extract (ASDE) in human endometriotic cells and rats.Method: Forty female rats were randomly divided into four groups (10 rats/group): control, endometriosis+danazol, endometriosis+high dose of ASDE and low dose of ASDE. The rats were orally administered either vehicle (200 μL of PBS) alone or ASDE (140, 280 and 560 mg/kg/day) for 5 weeks. Danazol was used as the control drug. After induction of endometriosis for 4 weeks, the rats were sacrificed by cervical dislocation and the peritoneum and visceral organs examined visually to measure the number of endometriotic lesions. Serum levels of cancer antigen 125 (CA-125) and interleukin 13 (IL-13), interleukin 18 (IL-18) and tumor necrosis factor-alpha (TNF-α) of peritoneal fluids of rats were measured using ELISA kits. Western blot assay was performed to measure the levels of matrix metalloproteinase-2 (MMP-2) and matrix metalloproteinase-9 (MMP-9) expressions after 24 h of treatment with ASDE (30, 60, and 120 μg/mL).Results: ASDE-treated rats displayed reduced numbers of total endometriotic lesions when compared with vehicle-treated controls (p < 0.01). When the rats were treated with high dose of ASDE, serum CA-125 level, as well as IL-18 and TNF-α levels in peritoneal fluids were significantly lower than that of the control group (p < 0.01); however, IL-13 level in peritoneal fluids was significantly higher than that of the control group (p < 0.01). ASDE treatment significantly suppressed the levels of MMP-2 and MMP-9 protein in 11Z cell (p < 0.01).Conclusion: The results reveal that ASDE exhibits significant anti-endometriotic effect by inhibiting inflammatory factors in rats. Thus, the plant extract can potentially be developed for the clinical management of endometriosis.
 Keywords: Angelica sinensis, Endometriosis, Cancer antigen, Endometriotic lesions, Matrix metalloproteinase
Highlights
Endometriosis is a common gynaecological disease that affects approximately 1 in 7 women and 30 - 50 % of infertile women [1]
Increased peritoneal fluid concentrations of cytokines that lead to the migration, proliferation, and activation of macrophages have been reported in patients with endometriosis
Angelica sinensis (Oliv.) Diels extract (ASDE) inhibited the formation of endometriosis-like lesions in rats
Summary
Endometriosis-associated inflammatory response, tissue repair and neo-vascularization are dependent on the peritoneal fluids macrophages and their secretary products/cytokines [4]. These cytokines may play major roles in regulating cell proliferation, activation, motility, adhesion, chemotaxis and morphogenesis in the pathogenesis of endometriosis [5]. Increased peritoneal fluid concentrations of cytokines that lead to the migration, proliferation, and activation of macrophages have been reported in patients with endometriosis. The anti-endometriotic effect of ASDE was investigated in human endometriotic cells and rats. The medicinal herbs of Angelica sinensis (Oliv.) Diels weighing 5 kilogram were collected from Guilin City, Guangxi Province in China in October 2018. For comparing more than two groups, one-way analysis of variance (ANOVA) was used followed by Tukey test with statistical significance set at p < 0.05
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